高效液相色谱法测定减肥降脂类中成药中非法添加化学药品洛伐他汀、盐酸西布曲明的探讨  

HPLC Determination of Lovatain and Sibutramine Hydrochloride Illegally Added in Chinese Patent Medicine for Reducing Weight and Fat

作　　者：刘莉[1] 程龙琼[1] 周世玉[1] 

机构地区：[1]成都市食品药品检测中心,610045

出　　处：《西南军医》2011年第4期599-601,共3页Journal of Military Surgeon in Southwest China

摘　　要：目的应用HPLC法测定减肥降脂类中成药中非法添加化学药品洛伐他汀、盐酸西布曲明。方法采用反相高效液相色谱法,Diamonsil(钻石)C18柱(5μm,4.6mm×200mm);流动相:0.025mol/L乙酸铵的0.1%乙酸溶液-乙腈(35:65));流速:1.0ml·min-1;测定波长:230nm;柱温:35℃。结果洛伐他汀进样量在3.084×10-2μg^3.084μg、盐酸西布曲明进样量在4.164×10-2μg^4.164μg范围内与峰面积成良好的线性关系,洛伐他汀与盐酸西布曲明平均回收率分别为98.25%、100.70%,RSD%分别为1.10%、0.92%。结论此方法简单,快速,准确,重复性好,适用于减肥降脂类中成药中洛伐他汀、盐酸西布曲明为指标的非法添加成分的检测。Objective To discuss the application of HPLC determination of lovatain and sibutramine hydrochloride illegally added in Chinese patent medicine for reducing weight and fat.Methods Rp-HPLC determination was performed on C18 column（5μm,4.6mm x 250mm）,the mobile phase was 0.1% acetic acid solution with 0.025mol/L ammonium acetate ─ acetonitrile（35：65）,the flow rate was 1.0ml/min,the detecting wavelength was 230nm and the column temperature was 35℃.Result Good linear correlation with the peak area was formed when the sample volume of lovatain was ranged between 3.084 x 102？g and 3.084？g and the sample volume of sibutramine hydrochloride between 4.164x 102？g and 4.164？g;the average yield of lovatain was 98.25%,RSD% was 1.1% while the average yield of sibutramine hydrochloride was 100.7%,RSD% was 0.92%.Conclusions This method is simple,fast,accurate and with good repeatability,it is suitable for the determination of lovatain and sibutramine hydrochloride illegally added in Chinese patent medicine for reducing weight and fat.

关 键 词：洛伐他汀 盐酸西布曲明 非法添加 HPLC 减肥降脂类中成药 

分 类 号：R286[医药卫生—中药学]