HPLC法同时测定复方板蓝根颗粒中的尿苷、腺苷和表告依春的含量  

Simultaneous Determination of Uridine,Adenosine and Epigoitrin in Compound Banlangen Granule by HPLC

作　　者：张慧晔[1] 罗杰[1] 黄亦南[1] 王德勤[1] 邓乔华[1] 李兰芳[1] 

机构地区：[1]广州白云山和记黄埔中药有限公司,广东广州510515

出　　处：《今日药学》2011年第6期337-339,共3页Pharmacy Today

摘　　要：目的建立复方板蓝根颗粒中尿苷、腺苷和表告依春的含量测定方法。方法采用Aglient XDB-C18分析柱（250 mm×4.6 mm,5μm）,流动相为：甲醇-0.2%磷酸水溶液,梯度洗脱,柱温为室温;流速0.7 m l/m in,检测波长254 mm;进样量20μl。结果尿苷、腺苷和表告依春分别在0.093 7~2.811 0μg（r=1.000）、0.060 9~1.827 0μg（r=1.000）、0.117 3~3.519 0μg（r=1.000）的范围内与峰面积呈良好的线性关系。加样回收率试验中尿苷、腺苷和表告依春的平均回收率分别为98.6%、99.6%、101.1%,RSD分别为0.6%、0.6%、0.8%。结论采用HPLC法同时测定复方板蓝根颗粒中的尿苷、腺苷和表告依春3种成分的含量,方法简便、快速,准确。Objective To establish a method for determination of uridine,adenosine and epigoitrin in compound Banlangen granule.Methods The determination was performed by HPLC with Aglient XDB-C18（250 mm × 4.6 mm,5 μm） column,methanol-0.2% phosphoric acid as mobile phase,wavelenghth at 254 nm and a flow rate of 0.7 ml/min.The column temperature was at room temperature.Results The linear range of uridine,adenosine,and epigoitrin were 0.093 7~2.811 0 μg（r=1.000）,0.060 9~1.827 0μg（r=1.000）,and 0.117 3~3.519 0 μg（r=1.000）,respectively.The average recovery of uridine,adenosine,and epigoitrin were 98.6%,99.6% and 101.1%,with RSD 0.6%,0.6%,and 0.8%,respectively.Conclusion The method is simple,rapid,and accurate.

关 键 词：复方板蓝根颗粒 尿苷 腺苷 表告依春 HPLC 

分 类 号：R284[医药卫生—中药学]