脑梗死早期侧支循环重建及电针干预效应研究  

Effect of electro-acupuncture and collateral circulation re-establish in early stage of cerebral infarction

作　　者：李桂平[1] 石磊[1] 杜元灏[1] 李颖[2] 

机构地区：[1]天津中医药大学第一附属医院,300193 [2]天津中医药大学,300193

出　　处：《天津中医药大学学报》2011年第2期102-104,共3页Journal of Tianjin University of Traditional Chinese Medicine

基　　金：国家自然科学基金资助项目(30672715)

摘　　要：[目的]以线栓法复制大鼠大脑中动脉梗死(MCAO)模型为研究对象,研究脑梗死急性期缺血半暗带侧支循环重建及电针的干预效应。[方法]造模后,电针干预组给予电针刺激,模型对照组不施加处理因素,于MCAO后1、3、6 h,股静脉注射番茄凝集素后行心脏灌注,取脑组织进行切片,染色,图像分析,数据统计,比较两组间有血流灌注的血管量变化。[结果]模型对照组和电针干预组在MCAO后13、、6 h有血流灌注的血管量明显下降,与空白对照组比较均存在统计学差异(P<0.001),电针干预组与模型对照组相应各时相比较,有血流灌注的血管量明显增加,组间比较存在统计学差异(P<0.05)。[结论]电针干预可增加MCAO模型大鼠缺血半暗带内有血流灌注的脑血管量,提示电针干预可促进侧支循环建立,从而增加这一区域的脑血流灌注,并对梗死区产生一定的影响。[Objective] To research the effect of the collateral circulation re-establish in semi-dark area and electro-acupuncture in the early stage of acute cerebral infarction ischemia in rats with MCAO induced by thread occlusion.The acute cerebral infarction in rat was induced by middle cerebral artery occlusion.[Methods] Using Lectin Histochemistry stain technique the changing of the blood vessel in the penumbra was observed and the effect of electro-acupuncture stimulus on blood vessel count at 1,3,6 hours after MCAO was evaluated.[Results] Lectin Histochemistry stain technique was used to reveal the morphological changing of the blood vessel After MCAO.Compared with normal brain,the blood vessel count were reduced in the penumbra（P0.001）.After electro-acupuncture stimulus,blood vessel count was raised in the peri-ischemic zone（P0.05） at 1,3,6 hours after MCAO.[Conclusion] Electro-acupuncture stimulus can significant increase the blood vessel count in the peri-ischemic zone and promote the establishment of collateral circulation after MCAO.

关 键 词：脑梗死 大脑中动脉梗死 侧支循环 电针 

分 类 号：R284[医药卫生—中药学]