水稻骨干亲本不同发育时期蛋白质表达的多态性  

The Profiling of Protein Expression at Different Developmental Stages of Elite Rice Varieties

作　　者：王宪云[1] 刘钊[1] 曹英豪[1,2] 白辉[2] 刘丽娟[1] 李莉云[1] 刘国振[1] 

机构地区：[1]河北农业大学生命科学学院,河北保定071001 [2]中国科学院北京基因组研究所,北京101300

出　　处：《中国农业科学》2011年第14期2849-2856,共8页Scientia Agricultura Sinica

基　　金：国家"973"基础研究项目(2006CB101705)

摘　　要：【目的】通过对水稻骨干亲本在不同发育时期的蛋白质表达特征研究,了解其形成机理并促进其应用。【方法】采用Western blotting调查了6个水稻骨干亲本(9311、培矮64s、特青、珍汕97B、广陆矮4号和矮脚南特)在不同生长发育时期/部位(苗期地上部与地下部、开花期剑叶和穗子、成熟期剑叶和穗子)与光合作用、活性氧清除及胁迫防御相关的9个蛋白质的表达。【结果】9个蛋白质的表达在不同骨干亲本间有多态性,尤其在地下部和穗子等部位的表达差异较大,而在叶片中的表达比较接近;此外,蛋白质表达在不同生长发育时期/部位间也有多态性,反映了蛋白质表达的时空特异性。【结论】初步建立一条利用蛋白质特异抗体了解水稻蛋白质表达的研究途径。[Objective] The characterization of protein expression at different developmental stages may help to understand the forming mechanisms of elite varieties and then promote their applications in breeding. [Method] In this study, Western blotting were used to investigate the expression of nine proteins, in the categories of photosynthesis, reactive oxygen scavenging, and defense/stress responses, among six rice elite varieties including 9311, PA64s, Teqing, Zhenshan 97B, Guangluai 4 and Aijiaonante, at different developmental stages/tissues including the shoot and root at seedling stage, the flag leaf and panicle at flowering stage, the flag leaf and panicle at filling stage. [Result] The results indicated that polymorphisms of protein expression presented among different rice varieties, the expression patterns in root and panicle varied to a large extent, while the expression in leaves was similar among different rice varieties. Additionally, the polymorphisms also presented among different developmental stages/tissues, indicating the temporal and spatials expressions of these proteins.[ Conclusion]This study has established a strategy to investigate the expression office proteins by using antibodies.

关 键 词：水稻 骨干亲本 蛋白质 多态性 

分 类 号：S511[农业科学—作物学]