根癌农杆菌介导mBt886Cry3Aa转化菊花及对菊天牛毒杀作用  

Transformed mBt886Cry3Aa into Chrysanthemum Mediated by Agrobacterium tumefaciens and Its Resistance to Phytoecia rufiventris Gautier

作　　者：王沛[1] 周洲[2] 苏丽娟[1] 尹新明[1] 

机构地区：[1]河南农业大学植物保护学院,郑州450002 [2]河南科技大学林学院,河南洛阳471003

出　　处：《中国农业科学》2011年第14期2918-2925,共8页Scientia Agricultura Sinica

基　　金：河南省杰出人才创新基金项目(074200510018);河南省重点科技攻关项目(092102110183)

摘　　要：【目的】将对鞘翅目害虫有毒杀作用的基因mBt886Cry3Aa转入菊花,并测定转基因菊花对靶标害虫—菊天牛的毒杀效果,为防治鞘翅目害虫提供新途径。【方法】以菊花为材料,确定茎段最适分化培养基和不定芽最佳生根培养基,卡那霉素最适筛选浓度,头孢噻肟钠最适抑菌浓度,通过农杆菌介导法完成mBt886Cry3Aa的遗传转化,利用转基因菊花与人工饲料混合对菊天牛2龄幼虫饲喂进行室内生物测定。【结果】经卡那霉素筛选和PCR检测,最终得到7株转基因菊花;选取长势健壮的4株转基因菊花混合人工饲料喂养菊天牛2龄幼虫,结果表明菊天牛2龄幼虫取食转基因菊花后的死亡率与对照幼虫(CK)死亡率差异显著。【结论】室内生物测定表明转基因菊花对菊天牛2龄幼虫具有较强的毒杀作用。[ Objective ] The objective of this study is to transform mBt886Cry3Aa into chrysanthemum, which has resistance to long-horned beetles, and determine the toxic effect of transgenic plants to Phytoecia rufiventris Gautier. The results of the study will provide a new way to control Coleoptera pests. [Method] mBt886Cry3Aa was transformed into chrysanthemum mediated by Agrobacterium tumefaciens according to the best culture media and the best suitable kanamycin and cefotaxime concentration confirmed for chrysanthemum differentiation and rooting, then artificial feed and transgenic plants were mixed to raise 2nd instar larvae. [ Result ] Seven transgenic plants including mBt886Cry3Aa were obtained by kanamycin riddling and PCR, and four strong transgenic plants mixed with artificial feed were used to raise 2nd instar larvae. The results showed that the mortality rates between transgenic plants and control were significantly different. [Conclusion] Bioassay indicated that the transgenic plants had better toxic effects on 2nd instar larvae.

关 键 词：菊花 菊天牛 再生体系 BT毒蛋白基因 生物测定 

分 类 号：S682.11[农业科学—观赏园艺]