菌株scl-2对苯线磷、哒嗪硫磷和丙溴磷降解的研究  

Biodegradation of pyridaphenthion,fenamiphos and profenofos by Arthrobacter sp.scl-2 and identification of the metabolites

作　　者：李荣[1] 王融[1] 杨秀娟[1] 陈启明[1] 宋瑶[1] 李顺鹏[1] 蒋建东[1] 

机构地区：[1]南京农业大学生命科学学院,农业部农业环境微生物工程重点开放实验室,江苏南京210095

出　　处：《中国环境科学》2011年第7期1178-1185,共8页China Environmental Science

基　　金：国家水体污染控制与治理科技重大专项(2009ZX07103-002);国家自然科学基金资助项目(31070100);江苏省环境保护厅科技项目(2009012)

摘　　要：研究了有机磷农药降解菌株Arthrobacter sp.scl-2对苯线磷、哒嗪硫磷和丙溴磷的降解特性,结果表明菌株scl-2可以利用苯线磷、哒嗪硫磷和丙溴磷为唯一磷源生长.菌株scl-2降解苯线磷、哒嗪硫磷和丙溴磷的最适温度均为30℃,哒嗪硫磷的降解在初始pH8时速度最快,而丙溴磷和苯线磷的最适初始pH均为7,偏酸偏碱均不利于菌株对苯线磷、哒嗪硫磷、丙溴磷的降解.利用气相色谱-质谱联机鉴定了苯线磷、哒嗪硫磷、丙溴磷的降解产物,分别为3-甲基-4-甲硫基苯酚、6-羟基-2-苯基-2-氢哒嗪酮和4-溴-2-氯苯酚.In this paper,the degradation characteristics of some organophosphorus_insecticides including pyridaphention,fenamiphos and profenofos by the isocarbophos-degrading strain Arthrobacter sp.scl-2were studied.It was found that strain scl-2could use pyridaphention,fenamiphos and profenofos as the sole phosphorus source for growth.The optimal temperature for the biodegradation of pyridaphention,fenamiphos and profenofos by strain scl-2was 30°C.The initial pH value for pyridaphention biodegradation was 8.0.However,the optimal pH values for fenamiphos and profenofos were found to be 7.0.Using the GC-MS technology,the metabolites of pyridaphention,fenamiphos and profenofos degraded by strain scl-2were identified to be 6-hydroxy-2-phenylpyridazin-3（2H）-one,3-Methyl-4-methylsulfanyl-phenol and 4-bromo-2-chlorophenol,respectively.

关 键 词：ARTHROBACTER sp.scl-2 苯线磷 哒嗪硫磷 丙溴磷 生物降解 

分 类 号：X703.5[环境科学与工程—环境工程]