4-硝基-4'-氟苯基重氮氨基偶氮苯与汞(Ⅱ)显色反应的双波长光度法测定的研究  

Study of color reaction between mercury(Ⅱ) and 4-nitro-4′-fluorobenzenediazoaminoazobenzene with dual-wavelength spectrophotometry

作　　者：俞善辉[1] 李四劼[1] 白云飞[1] 黄姗[1] 吴斌才[2] 

机构地区：[1]华东理工大学化学与分子工程学院,上海200237 [2]华东师范大学化学系,上海200062

出　　处：《化学试剂》2011年第7期631-633,662,共4页Chemical Reagents

摘　　要：研究了4-硝基-4'-氟苯基重氮氨基偶氮苯与汞(Ⅱ)的显色反应。实验表明,在Triton X-100存在下,pH 10.4的硼砂-氢氧化钠缓冲溶液中,汞(Ⅱ)能与显色剂发生显色反应,形成1:2的黄色稳定配合物。在485、425 nm处分别有最大正、负吸收峰。以485 nm为测量波长、425 nm为参比波长进行双峰双波长法测定,汞(Ⅱ)浓度在0～22μg/25 mL范围内符合比耳定律,表观摩尔吸光系数为1.89×105 L·mol-1·cm-1,加入混合掩蔽剂柠檬酸-酒石酸钠水溶液,用此法可测定工业废液中的痕量汞(Ⅱ),分析结果的相对标准偏差<3.0%,加标回收率在95.0%～97.5%之间。The color reaction between Hg（Ⅱ） and 4-nitro-4′-fluoro benzenediazoaminoazobenzene has been studied.In the presence of Triton X-100 and the borax-sodium hydroxide buffer solution of pH 10.4,color reaction happens between Hg（Ⅱ） and the chromogenic reagent,forming a yellow steady complex at 1∶2.The positive absorption summit is 485 nm,and the negative 425 nm.Dual wavelength test has been conducted,485 nm as measuring wavelength and 425 nm reference wavelength,Beer′s law being obeyed with Hg（Ⅱ） at 0～22 μg/25 mL,and the molar absorptivity is 1.89×105 L·mol-1·cm-1.Plus the mixed screening agents of citric acid-sodium tartrate aqueous solution,the approach can be employed to test the content of trace Hg（Ⅱ） in industrial waste water with RSD 3.0% and recovery of 95.0%～97.5%.

关 键 词：双波长分光光度法 4-硝基-4'-氟苯基重氮氨基偶氮苯 汞(Ⅱ) 

分 类 号：O657.32[理学—分析化学]