用微孔板分光光度法测定薯蓣皂苷元的含量  

Diosgenin Quantitative Analysis by Microplate Spectrophotometry

作　　者：李培琴[1] 阴春晖[1] 孟祥杰[1] 牟燕[1] 单体江[1] 周立刚[1] 

机构地区：[1]中国农业大学农学与生物技术学院,北京100193

出　　处：《天然产物研究与开发》2011年第3期486-489,共4页Natural Product Research and Development

基　　金：北京市自然科学基金(6092015);国家自然科学基金(30871662;31071710)

摘　　要：为了对植物样品中薯蓣皂苷元的含量进行高通量快速测定,本研究采用高压酸解制备薯蓣皂苷元,以高氯酸为显色剂,用微孔板分光光度法测定样品中薯蓣皂苷元的含量。合适的分析条件为:反应温度为30℃、高氯酸用量为200μL、振荡时间2 min后静置10 min,在410 nm处测定光吸收值。该方法的线性范围为每孔薯蓣皂苷元2～10μg(R=0.9988),平均回收率为99.9%,精密度的RSD为1.65%。该方法操作简单、准确稳定,可实现大批量样品中薯蓣皂苷元的快速检测。In order to quantitatively analyze diosgenin content of plant samples in large amounts in a short time,one method by microplate spectrophotometry with perchloric acid was established.Diosgenin was prepared from plant material by means of sulphuric acid hydrolysis at high pressure.The optimized analysis conditions were 30 ℃ of reaction temperature,200 μL of percholoric acid in each well of the plate,2 min of agitation plus 10 min of static status for the reaction,and detection at 410 nm of wavelength.The linear range for detection with this method was 2-10 μg of diosgenin in each well and its correlation coefficient（R） was 0.9988.The average recovery of diosgenin was 99.9%,and the relative standard derivation（RSD） of precision was 1.65%.This method was easy,accurate and stable,which was suitable for rapid determination of diosgenin content in a large number of samples.

关 键 词：薯蓣皂苷元 分光光度法 定量分析 微孔板 高压酸解 高氯酸 

分 类 号：R931.71[医药卫生—生药学] Q949.9[医药卫生—药学]