同种异体脂肪源干细胞移植对耐力训练大鼠血清生化指标及运动能力的影响  

Effect of allogenic adipose-derived stem cell grafting on serum biochemical indicators and sporting ability of endurance trained rats

作　　者：杨会营[1] 陶晖[1] 余美春[1] 杨春[1] 曲戎梅[1] 曾文钦[1] 段富华[1] 余磊[1] 戴景兴[1] 原林[1] 

机构地区：[1]南方医科大学人体解剖教研室暨广东省组织构建与检测重点实验室,广东广州510515

出　　处：《南方医科大学学报》2011年第7期1159-1163,共5页Journal of Southern Medical University

基　　金：国家重点基础研究计划(973计划)项目(2007CB512705);国家自然科学基金青年面上项目(30801464)~~

摘　　要：目的从筋膜学角度观察同种异体移植脂肪源干细胞(ADSCs)对耐力训练大鼠体内某些血清生化指标活性及运动能力的影响。方法 40只Wistar雄性大鼠随机分成安静对照组(空白对照组)、游泳训练照组(模型组)、安静移植细胞组(实验对照组)和游泳移植细胞组(实验组)。模型组和实验组进行力竭游泳训练。力竭游泳训练1周后,实验组与实验对照组经尾静脉输入ADSCs。游泳训练结束后,记录各组大鼠游泳力竭的时间以及检测血清尿素(BUN)、乳酸脱氢酶(LDH)、血乳酸(BLa)和血红蛋白(Hb)含量。结果模型组与空白对照组比较,Hb水平显著降低,血清BUN、LDH、BLa水平均显著升高(P<0.01),力竭时间显著延长(P<0.01);与模型组比较,实验组Hb显著提高(P<0.01),而BUN、LDH、BLa水平均明显降低(P<0.01),力竭时间有较大提高(P<0.01)。结论移植ADSCs可有效延缓了大鼠运动性疲劳的产生,提高了大鼠的运动能力。Objective To observe the effect of transplantation of allogeneic adipose-derived stem cells（ADSCs） on serum biochemical indicators and sporting ability in highly endurance-trained rats from a fasciological perspective.Methods The ADSCs were cultured in vitro.Forty male Wistar rats were randomized into 4 equal groups,namely the blank control group,overtraining（model） group,transplantation without training group and overtraining plus transplantation group.The rats in the two overtraining groups were subjected to exhaustive swimming for 1 week,and in the two transplantation groups,cultured allogeneic ADSCs（2 × 10 6 /ml） were injected via the tail vein.The exhaustion time in swimming and the serum levels of BUN,LDH,BLa,and Hb of the rats were recorded after the treatments.Results The rats in the model group showed significantly increased serum BUN,LDH and BLa levels and decreased Hb level with a extended exhaustion time as compared with those in the blank control group（P〈0.01）.The BUN,LDH and BLa was significantly lower,Hb level higher and the exhaustion time significantly longer in the overtraining plus transplantation group than those in the model group（P〈0.01）.Conclusions ADSCs can effectively prolong the exhaustion time of rats during exhaustive swimming and enhance their sporting ability.

关 键 词：脂肪源干细胞 同种异体移植 力竭游泳 筋膜学 运动能力 

分 类 号：R87[医药卫生—运动医学]