四株广东鸽源新城疫病毒的生物学特性  

Biological characterization of four pigeon Newcastle disease viruses isolated from Guangdong

作　　者：黎俊君[1] 何琴[1] 焦培荣[1] 刘大伟[1] 韦娜娜[1] 龚军[1] 廖明[1] 任涛[1] 

机构地区：[1]华南农业大学兽医学院农业部动物疫病防控重点开放实验室广东省动物源性人畜共患病预防与控制重点实验室,广东广州510642

出　　处：《中国兽医科学》2011年第7期677-683,共7页Chinese Veterinary Science

基　　金：公益性行业(农业)科研专项(200803020-08);国家自然科学基金项目(31072319)

摘　　要：采用RT-PCR方法从广东省分离的4株鸽新城疫病毒中扩增出F和HN基因片段,并对其进行克隆及序列分析,另外对该4株分离株进行了致病指数的测定。结果发现,分离株NDV/GD-1的静脉接种致病指数为1.84,脑内接种致病指数为1.287 5,鸡胚平均死亡时间为78h;NDV/GD-2的静脉接种致病指数为1.55,脑内接种致病指数为1.162 5,鸡胚平均死亡时间为138h;NDV/GD-3的静脉接种致病指数为0.44,脑内接种致病指数为1.35,鸡胚平均死亡时间为126h;NDV/GD-4的静脉接种致病指数为2.51,脑内接种致病指数为1.35,鸡胚平均死亡时间为84h;分离株NDV/GD-2属于基因Ⅱ型;NDV/GD-4属于基因Ⅶd型,与广西株Gxp40亲缘关系最近;NDV/GD-1、NDV/GD-3均属于基因Ⅵ型,与云南分离株Pigeon/China/YN亲缘关系最近;比较分离株与流行的新城疫基因Ⅶ型毒株的F全基因序列以及分离株与常用疫苗株的F、HN全基因序列,发现核苷酸和氨基酸差异较大,证实这4株分离株的生物学特性均有不同程度的差异。F and HN genes of four pigeon Newcastle disease viruses isolated from Guangdong were amplified by reverse transcription-polymerase chain reaction（RT-PCR） and sequenced.The pathogenicity of the four viruses were tested.The results revealed that the IVPI,ICPI and MDT of NDV/GD-1 were 1.84,1.287 5 and 78 h,respectively.The IVPI,ICPI and MDT of NDV/GD-2 were 1.55,1.162 5 and 138 h,respectively.The IVPI,ICPI and MDT of NDV/GD-3 were 0.44,1.35 and 126 h,respectively.The IVPI,ICPI and MDT of NDV/GD-4 were 2.51,1.35 and 84 h,respectively.NDV/GD-2 was belonging to genotypeⅡ.NDV/GD-4 was belonging to genotypeⅦ and close to Gxp40 from Guangxi of China.NDV/GD-1 and NDV/GD-3 were belonging to genotypeⅥ and close to Pigeon/China/YN from Yunnan of China.There were differences in nucleotide and deduced amino acid sequences of the F and HN gene with reference strains and vaccine strains,which showed that the biological characterization of these four pigeon Newcastle disease viruses were diversity.

关 键 词：鸽 新城疫病毒 生物学特性 

分 类 号：S852.659.5[农业科学—基础兽医学]