蜂胶黄酮对卡介苗联合脂多糖诱导小鼠免疫性肝损伤的保护作用  

The protective effect of propolis flavonoids on immunological liver injury in mice

作　　者：苏晓杰[1] 张建[1] 赵晶[2] 张悦[1] 

机构地区：[1]哈尔滨医科大学大庆校区医学检验与技术学院,大庆163319 [2]哈尔滨医科大学第五附属医院检验科

出　　处：《国际免疫学杂志》2011年第4期315-317,共3页International Journal of Immunology

摘　　要：目的探讨蜂胶黄酮（PF）对卡介苗（BCG）与脂多糖（LPS）诱导小鼠免疫性肝损伤的作用。方法除正常对照组外，采用BCG和LPS诱导小鼠建立免疫性肝损伤模型，随机分为5组，药物实验组分别用300mg／kg、150mg／kg、75mg／kg的蜂胶黄酮干预后，计算肝指数；检测血清中谷丙转氨酶（ALT）和谷草转氨酶（AST）活性；测定肝组织中丙二醛（MDA）、超氧化物歧化酶（SOD）和谷胱甘肽过氧化物酶（GSH—Px）等生化指标。结果蜂胶黄酮高、中剂量实验组小鼠肝指数下降，与模型组比较差异有统计学意义（F=9．88，P〈0．05）且不同程度地降低血清ALT及AST活性（F值分别为55．66和6．86，P〈0．05）；蜂胶黄酮各浓度组均能提高肝组织中SOD、GSH—Px水平（F值分别为11．96和11．14，P〈0．05），减少MDA的含量（F=8．61，P〈0．05）。结论蜂胶黄酮对BCG与LPS联合诱导的小鼠免疫性肝损伤具有一定的保护作用。Objective To investigate the protective effect of the propolis flavonoids on the BCG and LPS-induced immunological liver injury in mice. Methods Kunming mice were divided into control group, model group, bifendate group , low-dosage, middling-dosage, and high-dosage propolis flavonoids groups. On the first day, the model group , bifendate group and the protection groups were injected BCG from tail vein and then the protection groups were orally administered with propolis flavonoids （300 mg/kg, 150 mg/kg,75 mg/ kg） daily, and the bifendate group were orally administered with bifendate （ 150 mg/kg） daily. At the end of 12 day, all mice were injected with LPS via the tail vein except control groups. The mice were weighed 16h later. The serum alanine aminotransferase （ALT） and aspartate transaminase （AST） activities were determined and the mice were scarified to calculate liver somatic index . Liver homogenate were prepared and liver tissue malondialdehyde （MDA） content and erythrocuprein （SOD） and glutathione peroxidase （ GSH - Px） activities were tested. Results Propolis flavonoids groups reduced the somatic index （ F = 9. 88, P 〈 0.05 ）, and decreased serum ALT and AST activities of liver of mice compared with the model group （ F = 55.66 or 6.86, P 〈 0.05 ） and also decreased MDA content （ F = 8. 61, P 〈 0.05 ） , increased SOD and GSH-Px activities, compared with the model group （ F = 1 1.96 or 11.14, P 〈 0.05 . Conclusion Propolis flavonoids protected the BCG and LPS-induced immunological liver injury in mice.

关 键 词：蜂胶黄酮 免疫性肝损伤 保护作用 

分 类 号：R285.5[医药卫生—中药学]