黄芪联合鞘内移植间充质干细胞对大鼠缺氧缺血性脑损伤修复作用的研究  

Effect of Astrasalus Injection Combined with Intrathecal Transplantation of Rat Mesenchymal Stem Cells for Repairing Hypoxic-ischemic Brain Damage in Rats

作　　者：余勤[1] 李佩佩[2] 周丽萍[1] 宣晓波[2] 盛丽先[2] 郭莹[1] 林洁[1] 董晖[1] 王标[1] 

机构地区：[1]浙江中医药大学生物工程学院,浙江杭州310053 [2]浙江中医药大学第一临床医学院,浙江杭州310053

出　　处：《中华中医药学刊》2011年第7期1455-1458,共4页Chinese Archives of Traditional Chinese Medicine

基　　金：浙江省卫生厅资助项目(2006A092);浙江省新苗人才计划(2010R410046)

摘　　要：目的:研究黄芪注射液联合鞘内移植大鼠骨髓间充质干细胞(rMSCs)对大鼠缺氧缺血性脑损伤(HIBD)修复的影响。方法:115只Wistar大鼠随机分成假手术组、模型组、PBS对照组、黄芪注射液对照组、rMSCs治疗组和rMSCs加黄芪注射液治疗组。造模后3天治疗组鞘内注射rMSCs,或同时腹腔注射黄芪注射液,对照组鞘内注射PBS或腹腔注射黄芪注射液,造模后24天行Morris水迷宫实验检测大鼠学习、记忆能力,移植后1、4、7、14、21、28天分别取脑组织作病理学检查,并应用双标免疫组化检测经5-溴-2-脱氧嘧啶(BrdU)标记rMSCs的神经元特异性烯醇化酶(NSE)和胶质纤维酸性蛋白(GFAP)表达情况。结果:治疗组学习、记忆能力较对照组有显著改善(P<0.05),rMSCs加黄芪注射液治疗组第3天平均逃避潜伏期显著低于rMSCs治疗组(P<0.05)。脑组织病理切片显示rMSCs加黄芪治疗组较rMSCs治疗组脑组织水肿、神经细胞变性和坏死程度减轻更明显。双标免疫组化显示rMSCs移植后1天就出现在脑组织,并表达NSE与GFAP。rMSCs加黄芪治疗组BrdU阳性细胞数除移植后14天外均显著多于rMSCs治疗组(P<0.05),NSE阳性率移植后1、7、14天显著高于rMSCs治疗组(P<0.05),GFAP阳性率除移植后14天外均显著高于rMSCs治疗组(P<0.05)。结论:鞘内移植rMSCs是治疗大鼠HIBD的有效方法;黄芪注射液在体内有诱导rMSCs向神经细胞分化的能力,并有协同rMSCs促进大鼠HIBD修复的作用。Objective：To observe the effect of astrasalus injection（ASI） combined with intrathecal transplantation of rat mesenchymal stem cells（rMSCs） for repairing hypoxic-ischemic brain damage（HIBD） in rats.Methods：115 Wistar rats were randomly divided into 6 groups,named respectively by letters from A-F.Excepting 14 rats in Group A were sham-operated,the other 101 were hypoxic-ischemic（H-I） insulted,which were treated respectively with nothing,PBS,ASI,rMSCs and ASI＋rMSCs 3 days after H-I insult.Learning and memory abilities were assessed by Morris water maze 24 days after H-I insult.The pathological changes of bain were observed,expression of neuron-specific enolase（NSE） or glial fibers acid protein（GFAP） on rMSCs were showed by double-labeled immunohistochemistry（IHC） respectively 1,4,7,14,21,28 days after H-I insult.Results：Learning and memory abilities in Group E,F are improved better than that in Group B,C and D（P0.05）.As compared with Group E,the average escape latency in Group F is shorter with statistical significance at the 3rd day（P0.05）.HE staining shows that the tissue edema,neuron degeneration and necrosis in Group F are less serious.Double-labeled IHC shows there are more BrdU positive cells in Group F（P0.05） except the 14th day.The positive rate of NSE is higher in Group F at the 1st,7th,14th day（P0.05） as well as that of GFAP which is higher（P0.05） except the 14th day.Conclusion：Intrathecal rMSCs transplantation is an effective method for treating HIBD in rats;ASI has the capacity for promoting rMSCs migration,inducing them differentiating into neuron-like cells in vivo,assisting rMSCs to repair HIBD in rats.

关 键 词：黄芪注射液 间充质干细胞 缺氧缺血性脑损伤 鞘内移植 

分 类 号：R285.5[医药卫生—中药学]