紫杉中紫杉烷和双黄酮类成分的薄层色谱鉴别  

TLC identification of the taxanes and biflavonoids in Taxus cuspidata

作　　者：顾佼佼[1] 佟昕[1] 袁久志[1] 马晓嘉[1] 刘晓秋[1] 

机构地区：[1]沈阳药科大学中药学院,辽宁沈阳110016

出　　处：《中国医药导报》2011年第20期98-100,共3页China Medical Herald

摘　　要：目的:建立紫杉药材中紫杉烷类和双黄酮类成分的薄层色谱鉴别法。方法:以紫杉宁为对照品,石油醚-乙酸乙酯-丙酮-甲醇(8∶2∶2∶1)为展开剂系统,用硅胶G板鉴别紫杉烷类成分;以金松双黄酮和银杏双黄酮为对照品,石油醚-乙酸乙酯-甲醇(4∶1∶1)为展开剂系统,用聚酰胺薄膜鉴别双黄酮类成分。结果:本方法斑点清晰,重复性好,并可将紫杉区别于一些松、杉、柏类植物的枝叶。结论:本方法简便,快速,可用于紫杉药材的质量控制。Objective：To establish the TLC identification method of taxanes and biflavonoids in Taxus cuspidata.Methods：Taxanes identification method was presented on silica gel plate,with taxinine as reference substance,the system of a mixture of petroleum ether-ethyl acetate-acetone-methanol as developing solvent.And biflavonoids identification method was presented on polyamide film,with sciadopitysin and ginkegetin as reference substance,the system of a mixture of petroleum ether-ethyl acetate-methanol as developing solvent.Results：The established methods had good reproducibility and the spots were distinct.And the new methods could also distinguish Taxus cuspidata from some kindred plant such as Pinaceae,Taxodiaceae,Cupressaceae Plant.Conclusion：The methods are simple and fast,which can be used as quality control methods of Taxus cuspidata.

关 键 词：紫杉 薄层色谱鉴别 紫杉宁 金松双黄酮 银杏双黄酮 

分 类 号：R931.5[医药卫生—生药学]