补元聪脑汤对老年性痴呆大鼠行为学及凋亡相关蛋白Bcl-2、Bax表达的影响  

Influence of the BCD to AD Big Rat's Praxiology and the Expression of Apoptosis Related Protein Bax and Bcl-2 in Rats'Hippocampus

作　　者：陈民[1] 陈欢雪[2] 孙芳芳[1] 

机构地区：[1]辽宁中医药大学附属医院,辽宁沈阳110032 [2]中国医科大学,辽宁沈阳110001

出　　处：《中华中医药学刊》2011年第7期1463-1465,I0001,共4页Chinese Archives of Traditional Chinese Medicine

基　　金：辽宁省教育厅资助项目(20060551)

摘　　要：目的:探讨补元聪脑汤对老年性痴呆(AD)大鼠认知能力、海马区凋亡相关蛋白Bcl-2、Bax表达的影响及其治疗阿尔茨海默病的可能作用机制。方法:采用鹅膏蕈氨酸(IBO)+β淀粉样蛋白1-42等量复合注射液法复制AD大鼠模型;并应用Morris水迷宫法进行行为学检测;用免疫组化法测定海马区Bcl-2、Bax阳性神经元数的表达。结果:模型组与正常组相比,认知能力显著下降,海马Bax表达增多,Bcl-2表达减少,充分证明造模的成功;以补元聪脑汤进行中药干预,并设西药脑复康作为药物对照组,中药组和西药组均能显著改善大鼠的认知能力,抑制Bax的表达,上调Bcl-2的表达。结论:补元聪脑汤对AD大鼠脑神经细胞的保护作用可能是通过增强受损海马区神经元Bcl-2蛋白的表达,并减少Bax蛋白的表达。从而改善AD大鼠的学习记忆能力。Objective：Discuss to the influence of Buyuan Congnao Decoction（BCD） which is AD rat models recognize ability and the expression of apoptosis related protein Bax and Bcl-2 in rats＇hippocampus and the possible protection mechanism of the Alzheimer＇s Disease（AD）.Methods：To adopt IBO and Aβ 1-42 of the mixed solution injection to hippocampus establish AD rat models,and apply Morris water maze method to examine study and memory abilities on AD rat models,The expression of apoptosis related protein Bax and Bcl-2 in rats＇hippocampus were studied by immunohistochemistry method.Results：BCD can improve AD big rat recognition ability,repress Bax expression and raise the expression of Bcl-2.Model set and normal set compared,recognize ability is descend,hippocampus of Bax expression increase,and Bcl-2 expression decrease;treatment set and model set compared,recognize ability is remarkable improvement,hippocampus of Bax expresses fall off,the expression of Bcl-2 is moved up.Conclusions：The protection function of BCD which to the AD rats＇ brain nerve cells is possible that strengthen expression to damaged hippocampus neuron Bcl-2,and decrease to Bax.Thus the study memory of the AD rat is improved.

关 键 词：老年性痴呆 补元聪脑汤 认知功能 水迷宫 BCL-2 BAX 细胞凋亡 大鼠 

分 类 号：R285.5[医药卫生—中药学]