通腑汤对急性肺损伤大鼠肺组织CGRP的影响  

The influence of Tongfu Decoction on CGRP in Acute Lung Injury Rats Model

作　　者：刘建秋[1] 王雪慧[1] 李竹英[1] 

机构地区：[1]黑龙江中医药大学附属第一医院呼吸科,黑龙江哈尔滨150040

出　　处：《中华中医药学刊》2011年第7期1470-1472,共3页Chinese Archives of Traditional Chinese Medicine

基　　金：黑龙江省教育厅资助项目(11541328)

摘　　要：目的:观察通腑汤对急性肺损伤大鼠肺组织CGRP水平的影响,探讨通腑汤治疗急性肺损伤的作用机制。方法:选取健康Wistar大鼠180只,随机分为正常对照组、模型组、地塞米松组、中药低剂量组、中药中剂量组、中药高剂量组、30只/组。尾静脉注射LPS复制ALI模型,半小时后中药各剂量组灌胃、地塞米松组腹腔注射治疗,于治疗后第1、2、6h,每组每次各10只,处死大鼠。ELISA法检测肺组织中CGRP的含量。结果:模型组CGRP含量高于正常对照组(P<0.05);地塞米松组、中药各剂量组CGRP含量均低于模型组(P<0.05);中药低剂量组CGRP含量高于地塞米松组(P<0.05);中药中、高剂量组CGRP含量与地塞米松组无差异(P>0.05);中药中、高剂量组CGRP含量均低于中药低剂量组(P<0.05);中药高剂量组CGRP含量与中药中剂量组无差异(P>0.05)。结论:通腑汤可降低ALI大鼠肺组织中CGRP的含量,高剂量组与中剂量组疗效较好。Objective：To observe the effect on CGRP in ALI rat lung tissue,and to approach the mechanism of Tongfu Decoction anti-ALI.Method：180 Wistar rats were divided into 6 groups randomly：the normal control group,the model group,hexadecadrol group,Chinese herbal medicine low-dose group,medium-dose group,and high-dose group,30 rats each group.Copying ALI model by caudal vein injecting LPS,giving respectively the Chinese herbal medicine intragastric administration and Dexamethasone intraperitoneal injection half an hour later,and executing rats in 1,2,6 hour after treatment respectively,10 rats each time in each group.We detected CGRP contene in lung by ELISA method.Results：CGRP contents in the model group were higher than the normal control group（P0.05）;contents in hexadecadrol group and Chinese herbal medicine groups were lower than the model group（P0.05）;contents in Chinese herbal medicine low-dose group were higher than hexadecadrol group（P0.05）;Chinese herbal medicine medium-dose group、high-dose group and hexadecadrol group had no statistical significance（P0.05）;contents in Chinese herbal medicine medium-dose group and high-dose group showed lower than low-dose group（P0.05）;Chinese herbal medicine medium-dose group and high-dose group had no statistical significance（P0.05）.Conclusions：Tongfu Decoction could decrease CGRP in lung of ALI rats,medium-dose and high-dose group were better.

关 键 词：通腑汤 ALI CGRP 

分 类 号：R285.5[医药卫生—中药学]