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作 者:张炯炯[1] 吕先真 杨向平[3] 李跃军[1] 徐建泓[1]
机构地区:[1]浙江医学高等专科学校,浙江杭州310053 [2]浙江省磐安农业公司,浙江磐安322304 [3]浙江省人民医院,浙江杭州310024
出 处:《中华中医药学刊》2011年第7期1573-1575,共3页Chinese Archives of Traditional Chinese Medicine
基 金:浙江省科技厅科技计划资助项目(2009C32091);浙江省中医药管理局科技计划资助项目(2008CB024)
摘 要:目的:测定复方博落回生物碱溶液对玄参黑点球象成虫的毒力。方法:应用文献法提取博落回果实、烟叶中的生物碱;将博落回生物碱、烟碱和表面活性剂组成复方博落回生物碱溶液;采用喷雾法将不同浓度的复方博落回生物碱溶液施药于玄参黑点球象成虫;通过正交试验,分析复方博落回生物碱溶液中各组分的作用;用计算机软件SPSS 11.5进行统计学分析。结果:复方博落回生物碱溶液对玄参黑点球象成虫的LD50为27.003mg/L、95%可信区间为15.677~80.073mg/L。正交试验考察了各因素对玄参黑点球象毒力作用的影响程度,各因素的最佳组合是A2B1C2D2。结论:复方博落回生物碱溶液对玄参黑点球象成虫有毒杀作用。各组分的最佳组合和施药方案是博落回生物碱原液∶水为1∶4、10%烟碱溶液、10%吐温-80,对每只玄参黑点球象的受药量分4次进行连续喷雾。Objective:To determine the poisonous effect of the compound solution of Macleaya cordata alkaloids on Cionus tamazo Kono imagoes.Method:To use literature method to extract alkaloids from fruits of Macleaya cordata and tobacco leaf;To make the compound solution of Macleaya cordata alkaloids by mixing Macleaya cordata alkaloids,nicotine and surfactant;To adopt spraying method to fire the compound solution with different concentrations on Cionus tamazo Kono imagoes;To apply orthogonal trial to detect the function of each component group of the compound solution;To analyze statistically by the software of SPSS 11.5. Result:The effect of LD50 on Cionus tamazo Kono imagoes by the cmpound solution of Macleaya cordata alkaloids is 27.003mg/L,and 95% of the confidential interval shows 15.677~80.073mg/L.Every observing factor through the orthogonal trial influences the poisonous effect on Cionus tamazo Kono.The best formula of the factors is A2B1C2D2.Conclusion:The cmpound solution of Macleaya cordata alkaloid has an effect of wiping out Cionus tamazo Kono imagoes.The best prescription is 1∶4 ratio for the Macleaya cordata alkaloid dope and water,to make up the compound solution of Macleaya cordata alkaloid by 10% nicotine dope and 10% Tween-80 to spray upon the Cionus tamazo Kono by the set dose in four consecutive times.
关 键 词:玄参黑点球象成虫 复方博落回生物碱溶液 毒力测定
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