半夏泻心汤及其苦降药组HPLC指纹图谱的比较研究  

Comparative Study on High Performance Liquid Chromatographic Finger Printing of Pinelliae Decoction for Purging Stomach-Fire and Its Bitter Drugs for Purgation

作　　者：童荣生[1] 李晋奇[1] 彭成[2] 邹静[1] 张康[3] 钟承爽[3] 

机构地区：[1]四川省医学科学院四川省人民医院药剂科,四川成都610072 [2]成都中医药大学药学院,四川成都610075 [3]西华大学生物工程学院,四川成都610039

出　　处：《中华中医药学刊》2011年第7期1578-1581,共4页Chinese Archives of Traditional Chinese Medicine

基　　金：四川省中医药管理局资助项目(303005002194002)

摘　　要：目的:采用高效液相色谱法比较半夏泻心汤及其苦降药组指纹图谱的差别。方法:采用PhenomenexGemini C18 110A(5μm,250mm×4.6mm)色谱柱,柱温40℃,乙腈-0.05%三乙胺水溶液(pH 3.0)梯度洗脱,检测波长275nm,对半夏泻心汤及其苦降药组水提醇沉液的HPLC指纹图谱进行比较分析。结果:该方法精密度、稳定性和重复性良好,相似度均大于0.99,在半夏泻心汤中17个和其苦降药组共有的色谱峰峰面积占总峰面积98.18%。结论:苦降药组水提醇沉液和半夏泻心汤水提醇沉液HPLC指纹图谱有一定的差别,但差别不大。Objective：To make comparative study on high performance liquid chromatographic finger printing of Pinelliae Decoction for Purging Stomach-Fire and its bitter drugs for purgation.Method：The high performance liquid chromatographic finger printing of Pinelliae Decoction for Purging Stomach-Fire and its bitter drugs for purgation were estabilished and analyzed and compared in the following conditions：Phenomenex chromatographic column Gemini C18 110A（5μm,250mm×4.6mm）,column temperature 40℃,the gradient elution with acetonitrile and 0.05% triethylamine water solution（pH 3.0）,and detection wavelength of 275nm.Result：The precision and stability and reproducibility was accurate.The similar degree was exceeded 0.99.The 17 chromatographic peaks were owned by both of Pinelliae Decoction for Purging Stomach-Fire and its bitter drugs for purgation.Their peak area was 98.18% in the Pinelliae Decoction for Purging Stomach-Fire.Conclusion：The high performance liquid chromatographic finger printing of Pinelliae Decoction for Purging Stomach-Fire and its bitter drugs for purgation have small distinction.

关 键 词：半夏泻心汤 苦降药组 指纹图谱 

分 类 号：R284.1[医药卫生—中药学]