复方土茯苓颗粒提取、醇沉工艺研究  

Study on Extracting and Alcohol Precipitating Craft of Compound Tufuling Granules

作　　者：刘志刚[1,2] 邓伟杰[1] 郑鹏成[1] 孙维峰[1] 吴新荣[1] 

机构地区：[1]广州军区广州总医院药剂科,广东广州510010 [2]广州中医药大学,广东广州510405

出　　处：《中华中医药学刊》2011年第7期1594-1595,共2页Chinese Archives of Traditional Chinese Medicine

基　　金：广东省科技计划项目(2009B030801277);广东省中医药局建设中医药强省立项课题(2009287)

摘　　要：目的:建立复方土茯苓颗粒的提取、纯化工艺。方法:采用正交试验优化复方土茯苓颗粒提取、醇沉工艺;采用HPLC法测定提取物中牛膝含有的蜕皮甾酮。结果:确定提取工艺为第1次用8倍量水煎煮1h,第2次用7倍量水煎煮1h;醇沉工艺为将药液浓缩至0.5g/mL(相当于药材),边搅拌边加适量乙醇至浓度为60%,静置12h,分离上清液。结论:验证试验表明,该提取、醇沉工艺客观可行、稳定合理,可为工业化生产提供理论依据。Objective：To optimize the extracting and alcohol precipitating craft of compound Tufuling granules by Orthogonal experiment.Methods：Orthogonal experiment was applied to optimize the influence factors of extracting and alcohol precipitating;ecdysterone in Radix Achyranthis Bidentatae of compound Tufuling granules concrete was determined by HPLC.Results：The best extraction craft is that the herbs should be extracted by 8 times（V/V） water for 1h,then be extracted by 7 times water for 1h;The best alcohol precipitating craft is the concrete should be concentrated to 0.5g/mL（counted by herbs）,added alcohol to 60%（V/V） with vigorously stirring,then placed for 12h and centrifugalize the supernatant fluid.Conclusion：Proving trial showed that this extracting and alcohol precipitating craft is scientific and reasonable,and can offer theory basic for industrialized manufacture of compound Tufuling granules.

关 键 词：复方土茯苓颗粒 正交试验 蜕皮甾酮 HPLC 

分 类 号：R284.2[医药卫生—中药学]