辽宁、吉林和朝鲜三地人参对脾虚小鼠细胞凋亡的影响  

Liaoning,Jilin and North Korea Three Ginseng Spleen Cells of Mice

作　　者：刘丽[1] 关洪全[1] 孙宏伟[1] 马贤德[1] 陈殿学[1] 

机构地区：[1]辽宁中医药大学,辽宁沈阳110847

出　　处：《中华中医药学刊》2011年第7期1627-1629,共3页Chinese Archives of Traditional Chinese Medicine

基　　金：辽宁省教委资助项目(2008456)

摘　　要：目的:探讨辽宁、吉林和朝鲜三地人参对小鼠脾细胞凋亡的影响并对三地人参的药效进行比较。方法:将80只昆明种小鼠随机分为空白对照组、脾虚模型组、正常三地人参灌胃组和脾虚三地人参治疗组,共8组,每组10只。空白对照组灌服生理盐水;脾虚模型组及脾虚三地人参治疗组以番泻叶浸泡液灌胃建立脾虚模型,模型建立后,脾虚三地人参治疗组及正常三地人参灌胃组分别以辽宁、吉林和朝鲜三地人参煎剂灌胃。给药14天后,流式细胞仪测定各组小鼠的脾细胞凋亡率。结果:与空白对照组比较,正常三地人参灌胃组小鼠的脾细胞凋亡率均有不同程度的提高,但没有统计学意义(P>0.05);正常三地人参灌胃组间比较差异不显著(P>0.05);与空白对照组比较,脾虚模型组的脾细胞凋亡率明显下降,两者间有统计学意义(P<0.05);与模型组比较,辽宁和吉林人参治疗组结果显著,有统计学意义(P<0.05);与模型组比较朝鲜人参治疗组治疗效果不显著,两者无统计学意义;三地人参治疗组间比较无统计学意义(P>0.05)。结论:辽宁、吉林和朝鲜三地人参对正常小鼠的脾细胞凋亡率无明显影响;脾虚状态下机体的脾细胞凋亡率降低,辽宁、吉林人参可使其提高至正常水平,朝鲜人参对其影响不显著;三地人参对脾虚小鼠脾细胞凋亡的影响相似,无产地差异。Objective：Of Liaoning,Jilin and North Korea Ginseng three apoptosis of mouse spleen cells and three to compare the efficacy of ginseng.Methods：The 80 Kunming mice were randomly divided into control group,spleen model group,the normal three groups and fed ginseng ginseng spleen three treatment groups,a total of 8 groups of 10 each.Control group,normal saline;spleen deficiency model group and treatment group three senna soaking ginseng orally established spleen model,model building,the spleen three ginseng treatment group and three groups were fed with ginseng Liaoning,Jilin and North Korea three ginseng decoction gavage.14 days after administration,the mice were measured by flow cytometry of spleen cell apoptosis.Results：Compared with the control group,normal mice fed the three ginseng spleen cell apoptosis rate was increased to varying degrees,but not statistically significant（P0.05）;normal three ginseng was no significant difference between the groups fed（P0.05）;compared with the control group,model group,the spleen spleen cells was significantly decreased,with statistical significance（P0.05）;with the model group,Liaoning and Jilin ginseng treatment group results were significantly（P0.05）;Korean ginseng with the model group was not significant between treatment groups;three ginseng treatment groups was not significant（P0.05）.Conclusion：Liaoning,Jilin and North Korea Ginseng three spleen cells of normal mice had no effect on apoptosis;spleen spleen cells of the body under the apoptosis rate decreased,Liaoning,Jilin Ginseng can be raised to its normal level,the Korean ginseng for its not significant;three ginseng on spleen cells of mouse spleen similar.

关 键 词：人参 脾虚 细胞凋亡 流式细胞仪法 

分 类 号：R285.5[医药卫生—中药学]