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作 者:王俊娟[1] 王德龙[1] 樊伟莉[1] 宋贵方[1] 王帅[1] 叶武威[1]
机构地区:[1]中国农业科学院棉花研究所/农业部棉花遗传改良重点实验室,河南安阳455000
出 处:《生态学报》2011年第13期3720-3727,共8页Acta Ecologica Sinica
基 金:中央级公益性科研院所基本科研业务专项(SJA1001);国家转基因重大专项(2008ZX08005-004);"十一五"国家科技支撑计划(2006BAD13B04-1);国家高技术研究发展计划(863计划)(2009AA101104)
摘 要:土壤盐渍化是一个世界性的资源问题和生态问题,盐分胁迫几乎会影响棉花所有重要的生命活动,造成棉花的减产或其他不利影响。通过对14个棉花品种(系)的萌发期耐盐性、芽期耐盐性以及两个耐盐性不同的品种(系)三叶期的耐盐性,对棉花不同时期的耐盐特性进行了研究,结果表明:棉花在不同生育阶段耐盐能力是不同的,棉花盐敏感时期是在萌发出苗期。随着生育期的延长,棉花的耐盐性是逐步提高的,进行耐盐性鉴定的最佳时期在是萌发出苗期。在三叶期,随着盐分浓度的提高,棉花苗受伤害程度逐渐增加,盐敏感品种(系)受伤害大于耐盐品种,在同样盐浓度胁迫条件下,耐盐性不同的品种各部位受到的伤害不同,耐盐品种(系)中棉所35受伤害程度由高到低依次为:真叶>上胚轴>子叶;盐敏感品种(系)Tam cot CAB-CS受伤害程度由高到低依次为:真叶>子叶>上胚轴。Soil salinity is a serious threat to cotton production worldwide,especially in arid and semi-arid regions.Excessive salts in the soil affect all most the whole living process of a cotton plant mainly through osmotic stress,iron toxity and nutrient imbalance,and finally leads to reduced cotton yield and fiber quality.Plant response to salinity stress may vary with genotypes and the growth stages in which the stress is imposed.However,it is still not clear if there exist difference in salt tolerance among current cotton cultivars at germination and seedling stages.The main objective of this study is to determine the salt tolerance of different types of cotton varieties.We attempted to develop easy and accurate methods for identifying cotton salt tolerance and screening genetic materials of cotton,with focus on difference in salt-tolerance of cotton between salt-tolerant cultivars and salt-sensitive cultivars at different stages during stand establishment.To achieve this objective,we conducted box and pot experiments at the Cotton Research Institute(CRI),Anyang,Henan province in 2009.Seeds of 14 cotton varieties(lines)(sGKzhong980,Lumianyan21,CRI35,Lumianyan16,CRI 45,Jimian668,DPlcon215,Handan109,Zhong404A,Yumian21,CRI12,Yinshuangjia,Zhong9806 and Line44) were grown in either a box for germination or in a pot containing saline soil for emergence.The germination rate and the leaf leakage solution electrical conductivity were determined.Two typical varieties(CRI35 and Tamcot CAB-CS) with different salt tolerance were also studied at three-leaf age stage.For the germination stage study,the cotton seeds were planted in the mixture of NaCl and sterilized sand,and incubated in a growth chamber.For the juvenile seedling stage study,the seedling germinated on wet double layers filter paper towel were transferred to the sterilized sand mixture which contained 0.4% NaCl(w/w),and incubated in a growth chamber.For the three-leaf stage,the seedlings were grown in the non-salt soil pots in a growth chamber and
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