南苜蓿高效共生根瘤菌土壤的筛选  

Screening of highly-effective rhizobial strains on Alfalfa (Medicago polymorpha) in soil

作　　者：刘晓云[1] 郭振国[1] 李乔仙[2] 刘桂霞[1] 薛世明[2] 王易鹏[3] 

机构地区：[1]河北大学生命科学学院,河北省微生物多样性研究与应用重点实验室,河北保定071002 [2]云南省草地动物科学研究院,云南昆明650212 [3]西南林学院保护生物学院,云南昆明650224

出　　处：《生态学报》2011年第14期4034-4041,共8页Acta Ecologica Sinica

基　　金：云南省应用基础研究计划面上项目(2007C067M)

摘　　要：利用云南德宏盈江县3种类型的土壤,对分离自云南楚雄、德宏等地的SWF67523、SWF67409、SWF67456等12株根瘤菌菌株进行了南苜蓿接种效果的研究。结果表明,在所有供试菌株中,菌株SWF67523、SWF67409、SWF67394表现最为优良,结瘤率较高的菌株是SWF67523、SWF67501、SWF67394和SWF67350,结瘤率均达到95%以上;而菌株SWF67523、SWF67409和SWF67394对植株株高影响最大;菌株SWF67409、SWF67523和SWF67394对提高植株干重贡献最大,其中菌株SWF67409比空白对照增产106.5%;供试菌株对含氮量的影响也很显著,接种菌株SWF67409、SWF67523和SWF67394的苜蓿植物含氮量相比其他菌株较高,影响显著。综合以上结果,发现来源于盈江南苜蓿的根瘤菌菌株SWF67523较其他菌株表现突出,同时发现根瘤菌菌株的接种效果受土壤因子的影响,在含有效钾中等的土壤平原镇Ⅱ上,接种根瘤菌的南苜蓿地上部分干重普遍高于其他两种有效钾含量为较缺的土壤,说明根瘤菌菌株与宿主和土壤环境存在密切关系,在适合的宿主和生活环境中将会发挥最大的共生固氮作用。Alfalfa（Medicago polymorpha） is an annual herbal plant in the pea family Fabaceae,has the highest feeding value as forage for its soft and highly digestible fiber and high protein content fiber up to 24.5%.Therefore,it recommended to cultivate widely and productively as an ideal forage plant.Inoculated with rhizobia,alfalfa（Medicago polymorpha） can formed effective symbiotic system to fix nitrogen,and subsequently produce high plant growth dry weigh.The inoculation effect on alfalfa（Medicago polymorpha） was performed in three different type of soils from Yingjiang county of Yunnan province by using twelve distinct rhizobial strains such as SWF67523,SWF67409,SWF67456,SWF67394,SWF67501,etc.respectively which were isolated from root nodules of Medicago polymorpha,M.sativa and M.lupulina from Yingjiang and other area of Yunnan.The results showed that the rhizobial nodulation rate,plant height,dry weight and total nitrogen content of plant were all increased significantly after harvested in condition that plant were inoculated with rhizobia（P0.05）,the rhizobial nodulation rate are up to 98.41%,97.78%,97.50% and 96.56% by inoculated with strain SWF67523,SWF67501,SWF67394 and SWF67350,respectively;Contrasting to the control,the plant height increasing 40.2%,27.3% and 25.0% by SWF67523,SWF67409 and SWF67394 respectively;The dry weight per plant was raised up 106.5% by inoculated with SWF67409 and raised 100.1%,78.3% and 74.3% by the other strains SWF67523,SWF67394 and HBU07001 respectively;Besides,the inoculated strains increased the nitrogen content obviously as well,the alfalfa plant have higher nitrogen content by inoculated with strain SWF67409,SWF67523 and SWF67394 than other test strains.Among 12 strains grew in three types of soils revealed by the whole production of the plant inoculation,strain SWF67523,SWF67409 and SWF67394 were more effective than others;Of the three strains,the performance of indigenous strain SWF67523 from its host of alfalfa（Medicago polymorpha） grew in Yinjiang exceeded

关 键 词：根瘤菌 南苜蓿 土壤 筛选 植物干重 

分 类 号：S541.9[农业科学—作物学]