甜菜夜蛾核多角体病毒Se62基因的克隆与结构分析  

Cloning and Sequence Analysis of Spodoptera exigua Multicapsid Nucleopolyhedrovirus Se62 Gene

作　　者：李赛男[1] 李充璧[1] 

机构地区：[1]肇庆学院生命科学学院,广东肇庆526061

出　　处：《安徽农业科学》2011年第18期10922-10925,10928,共5页Journal of Anhui Agricultural Sciences

基　　金：肇庆学院自然科学青年项目(0649)

摘　　要：[目的]克隆并分析甜菜夜蛾核多角体病毒(Spodoptera exigua multicapsid nucleopoledrovirus,SeMNPV)ORF62全长基因(Se62)。[方法]采用PCR的方法扩增Se62基因,将其克隆至pMD18-T载体上,获得了重组质粒T-Se62,进行序列测定和分析。[结果]获得大小为1 128 bp左右的序列,扩增序列与GenBank登录序列(No.NC_002169)核苷酸同源性100%。序列分析表明,Se62基因编码蛋白(SE62)存在明显的跨膜区域,有多个蛋白激酶C、酪蛋白激酶Ⅱ磷酸化位点和N-糖基化位点。蛋白序列比对结果表明,SE62属于杆状病毒P48蛋白超级家族成员,与苜蓿丫纹夜蛾核多角体病毒(Autographa califorica multicapsid nucleopoledrovirus,AcMNPV)P48蛋白的同源性为52%,其在病毒复制中的作用值得关注。[结论]成功克隆出Se62基因,为研究其在病毒复制中的功能奠定了基础。[ Objective] The aim was to obtain and analyze Se62 gene from Spodoptera exigua multicapsid nucleopolyhedrovirus （SeMNPV） ge- nomic DNA. [ Method] The sequence of Se62 gene was obtained by PCR method. The PCR product was cloned into pMD18-T vector to get the re- combinant plasmid （T-Se62）. Then Se62 gene was sequenced and analyzed. [ Result ] Nucleotide sequence analysis showed that the amplified se- quence was 1 128 bp in length,shared 100% identity with the sequence of GenBank （No. NC_002169） of Se62. Predicted protein from nucleo- tide sequence of Se62 （SE62） had two notable transmembrane regions,and contained much PKC-PHOSPHO-SITE, CK2-PHOSPHO-SITE, ASN_ GLYCOSYLATION. BLAST protein results showed that SE62 belonged to baculo_P48 protein super family and had high homology with Autogra- pha califorica multicapsid nucleopolyhedrovirus （AcMNPV） 1748 （Ac_P48） （52%）. The function of SE62 in the course of virus production claims attention. [ Conclusion ] Se62 gene was cloned,which laid a foundation for further researeh of the gene functions in the course of virus pro- duction.

关 键 词：甜菜夜蛾核多角体病毒 Se62 克隆 序列分析 

分 类 号：S433.4[农业科学—农业昆虫与害虫防治]