纤维素同步糖化发酵生产乙醇  

Research on the Ethanol Produced from the Simultaneous Glycation and Fermentation of Cellulose

作　　者：孙武举[1] 翁海波[1] 李萍萍[1] 晋果果[1] 

机构地区：[1]郑州大学生物工程系,河南郑州450001

出　　处：《安徽农业科学》2011年第18期11018-11021,11126,共5页Journal of Anhui Agricultural Sciences

基　　金：河南省重点科技攻关资助项目(072102220002)

摘　　要：[目的]利用微生物方法生产乙醇,从而替代化石能源。[方法]土曲霉M11利用纤维素为原料产酶并糖化纤维素成还原糖,利用酿酒酵母发酵生成乙醇。[结果]通过对土曲霉M11生长条件的研究,确定了土曲霉M11的最佳培养时间是3 d,最佳接种量为200μl,最适培养湿度为80%,最适培养温度为45℃,最适培养pH为3.0,此条件下可获得最大的产酶量。通过对糖化过程的研究,确定了纤维素酶的最适糖化温度为55℃,最适pH为5.0,此条件下可获得较高的还原糖量,且在酸性条件下酶活力较高,具有很好的热稳定性。通过发酵,还原糖量占原材料干重的62.42%,产生的乙醇占原材料干重的21.36%。[结论]此方法可以应用于工业发酵生产乙醇,有利于保护环境、降低成本、提高社会效益,有很好的应用价值。[Objective] The ethanol was produced by means of microbiological processes for the replaeing approach of energy source. [ Method ] The reducing sugar was produced from the cellulose ,which was saccbarified by the enzyme that was from the cellulose as raw material was acted by Aspergillus terreus--M11, and the ethanol was produced based on the fermentation of yeast. [ Result ] The experimental result indicated that the optimal culture eondition of Aspergillus terreus-M11 growth was that the best time was 3 days, the best inoculation was 200 μl, the optimal culture humidity was 80% ,the optimum temperature was 45 ~C and the optimal culture pH was 3.0 ,under which condition ,the largest amount of enzyme- producing was available. And the experiment in the glycation process of cellulase showed the optimal temperature was 55 ℃ and the optimum pH was 5. O, under which condition the production of reducing sugar, which enzyme activity under acidic condition was higher and had good thermal stability, was relevantly high. By fermentation, the reduced sugar accounted for 62.42% of the dry weight of raw material and the produced ethanol accounted for 21.36% of dry weight of raw material. [ Conclusionl This method could be used for the production of ethanol at the basis of indus- trial fermentation with the environmental protection ,cost-reducing and high social benefit,which application value was very good.

关 键 词：同步糖化发酵 土曲霉M11 乙醇 废纸 酶活性 

分 类 号：Q93[生物学—微生物学]