水貂MLPH基因第一外显子序列结构及特征分析  

Sequence Structure and Characteristics of Exon 1 of Mink MLPH Gene

作　　者：张敏[1] 

机构地区：[1]辽宁医学院锦州畜牧兽医学院,辽宁锦州121001

出　　处：《安徽农业科学》2011年第18期11097-11099,共3页Journal of Anhui Agricultural Sciences

基　　金：辽宁医学院博士启动资金资助

摘　　要：[目的]克隆辽西地区饲养水貂的MLPH基因第一外显子序列及其侧翼序列,预测其结构特征,为培育彩色水貂提供理论依据。[方法]扩增水貂MLPH基因第一外显子和5'侧翼序列,利用SignalP 3.0 Server、ClustalX 1.83、Mega 4.1、TMHM 2.0、ProtComp-v6.0等生物学软件进行序列分析。[结果]测序得到1 029 bp长的序列片段,利用Mega4.1软件计算水貂与大家鼠的遗传距离是最近的0.540 5,与人的遗传距离是最远的0.655 2,构建水貂分子系统进化树。[结论]水貂MLPH基因是一个分泌蛋白。[ Objective ] To clone exon 1 and its flanking sequences of MLPH gene from minks raised in western Liaoning region and to predict its structure. The study will provide a theoretical basis for breeding of colored mink. [Method] The exon 1 and its 5＇-flanking sequences of MLPH gene were amplified from minks. SignalP 3.0 Server, ClustalX 1.83, Mega 4.1, TMHM 2.0, ProtComp - v6.0 and other software were used for se- quence analysis. [ Result] A 1 029 bp fragment was amplified with the expected size. As calculated with the Mega 4.1 ,the minks had the nearest genetic distance with rattus （0.540 5 ） and the farthest with human （0. 655 2）. Phylogenetic tree of mink was constructed. [ Conclusion ] The Mink MLPH gene encodes a secretory protein.

关 键 词：水貂 MLPH基因 序列结构 

分 类 号：S821.3[农业科学—畜牧学]