水稻水通道蛋白OsPIP2-6的亚细胞定位研究  

Subcellular Localization of Aquaporins OsPIP2-6 in Rice

作　　者：姚清国[1] 李晓芹[1] 李小兵[1] 韩爱云[1] 

机构地区：[1]石家庄学院化工学院,河北石家庄050035

出　　处：《安徽农业科学》2011年第17期10108-10108,10115,共2页Journal of Anhui Agricultural Sciences

基　　金：2010年石家庄市科学技术研究与发展项目(10120803);2007年石家庄学院科研启动基金立项(2007012)

摘　　要：[目的]探讨水稻水通道蛋白OsPIP2-6的亚细胞定位。[方法]克隆了水稻中的重要通道蛋白OsPIP2-6基因,构建了瞬时表达载体,通过基因枪法在洋葱表皮中进行瞬时表达,并通过激光共聚焦显微镜进行了观察。[结果]水稻水通道蛋白OsPIP2-6主要定位在细胞质膜上,证明了OsPIP2-6是个水通道蛋白。[结论]为植物水通道蛋白的深入研究提供了理论依据。[ Objective ] This study was to explore the subcellular localization of aquaporins OsPIP2-6 in rice. [ Method ] A key rice aquaporins gene OsPIP2-6 was cloned and used for construction of a transient expression vector, which was then transformed into onion epidermis via par- ticle bombardment for confocal microscopy analysis using YFP gene as a reporter gene. [Result] The results showed that rice aquaporins OsPIP2-6 was mainly located in the plasma membrane. [ Conclusion] Our results provided theoretical basis for further understanding plant aquaporins.

关 键 词：水通道蛋白 水稻 亚细胞定位 

分 类 号：Q291[生物学—细胞生物学]