纹枯病菌诱导水稻表达的WRKY基因克隆及分析  

Cloning and Analysis of WRKY Gene of Rice Induced by Rhizoctonia solani Kühn

作　　者：姜述君[1,2] 马建[2] 范文艳[2] 戴凌燕[1] 张国庆[2] 于涵[2] 刘朝[2] 

机构地区：[1]黑龙江八一农垦大学生命科学技术学院,黑龙江大庆163319 [2]黑龙江八一农垦大学农学院,黑龙江大庆163319

出　　处：《安徽农业科学》2011年第17期10112-10115,共4页Journal of Anhui Agricultural Sciences

基　　金：黑龙江省高等学校青年学术骨干支持计划项目(1152G022)

摘　　要：[目的]克隆纹枯病菌诱导的水稻上调表达基因。[方法]对应用抑制差减杂交技术(SSH)获得的纹枯病菌诱导水稻表达的EST序列K16进行电子克隆,根据电子克隆产物设计引物进行RT-PCR、克隆测序,利用生物信息学技术对克隆产物进行功能预测。[结果]克隆出一个长度为1079bp的序列,结构功能分析显示该基因编码236个氨基酸的蛋白,存在多个功能位点,含有2个典型的WRKY结构域和1个C2H2锌指型结构,该基因与水稻WRKY8、WRKY24和WRKY30基因有较高的同源性。[结论]经电子克隆获得的纹枯病菌诱导表达的基因为典型的WRKY家族基因,该基因可能在水稻抗纹枯病中起重要作用。[ Objective ] The aim was to clone the up-regulated expression gene of rice induced by Rhizoctonia solani. [ Method ] The EST frag- ment KI6 obtained by suppression subtraction hybridization （SSH） was cloned and confirmed by reverse transcription-polymerase chain reac- tion （RT-PCR）. Then RT-PCR products were cloned into the PMD18-T vector and sequenced. The functions of the sequence were predicted with bioinformatics method. [ Result] A 1 079 bp gene was obtained. The gene encoded a protein with 236 amino acids. The protein contains many motif sites, two WRKY domains and a C2H2 zinc finger motif. The gene showed high identities with WRKYS, WRKY24 and WRKY30 gene of rice. [ Conclusion] The up-regulated expression gene induced by R. solani was representative WRKY family gene. The gene could play an important role on rice sheath blight resistance.

关 键 词：水稻 水稻纹枯病菌 电子克隆 WRKY基因 

分 类 号：S435.111.4[农业科学—农业昆虫与害虫防治] Q7[农业科学—植物保护]