广西地区甘蔗黄叶病病毒分子鉴定  

Molecular Identification of Sugarcane Yellow Leaf Virus in Guangxi

作　　者：颜梅新 黄伟华 黄诚华 

机构地区：[1]广西甘蔗研究所广西甘蔗遗传改良重点实验室,广西南宁530007

出　　处：《安徽农业科学》2011年第17期10156-10156,10163,共2页Journal of Anhui Agricultural Sciences

基　　金：现代农业产业技术体系建设专项资金项目(nycytx-024-01-07);广西自然科学基金项目(2010GXNSFB013026);广西农业科学院基本科研专项(G2010010;201004)

摘　　要：[目的]为广西甘蔗黄叶综合征(YLS)的分子检测和抗病育种提供参考。[方法]采集显症甘蔗叶样本,通过提取总RNA,利用引物P1/P2对样本进行反转录RCR(RT-PCR)扩增,对广西地区甘蔗黄叶病的病原体进行分子鉴定。[结果]经引物P1/P2扩增出630bp的目的片段。氨基酸和核苷酸序列分析结果表明,该片段是甘蔗黄叶病毒(SCYLV)外壳蛋白基因的一部分,与其他国家SCYLV分离物同源性达95%～99%。[结论]广西甘蔗黄叶病病原确是SCYLV,该病毒可能是近年从区外或境外传入的。[ Objective ] The reference for the molecular detection of sugarcane yellow leaf（YSL） and the disease-resisting breeding of sugar- cane in Guangxi was provided through the research. [ Method ] Total RNA, extracted from the sample with the disease symptom, was amplified with specific primers P1/P2 for RT-PCR. [ Result] A distinct fragment of about 630 bp was amplified. The analysis of the nucleotide sequence of the RT-PCR product was found it was the part the SCYLV, which was 95 % -99% identical to sequence of the SCYLV isolated from other countries. [ Conclusion] The virus infecting the sugarcane in Guangxi was identified as sugarcane yellow leaf virus（SCYLV）.

关 键 词：甘蔗 黄叶病 甘蔗黄叶病毒 分子鉴定 

分 类 号：S132[农业科学—农业基础科学]