水稻硫转运蛋白基因OsST的亚细胞定位  

Subcellular Localization of the Sulfate Transporter Gene OsST in Rice (Oryza sativa L.)

作　　者：王雪艳[1] 潘露琪[1] 楼依哲[1] 葛颖慧[1] 赵海军[2] 

机构地区：[1]中国计量学院生命科学学院,浙江杭州310018 [2]浙江大学原子核农业科学研究所/农业部核农学重点开放实验室,浙江杭州310029

出　　处：《安徽农业科学》2011年第17期10190-10191,10332,共3页Journal of Anhui Agricultural Sciences

基　　金：国家自然基金青年基金项目(NSFC30900887);中国博士后基因特别资助项目(2009020654);面上资助项目(20080441264)

摘　　要：[目的]构建水稻硫酸根转运基因OsST与YFP黄色荧光蛋白融合表达载体,对OsST蛋白进行亚细胞定位。[方法]从水稻叶片的cDNA中克隆OsST基因ORF全长,测序验证后连入pA7-YFP表达载体,通过基因枪将融合载体转入洋葱上表皮细胞,在激光共聚焦显微镜下观察细胞中荧光发光部位。[结果]OsST蛋白定位于细胞膜和核膜上。[结论]为进一步研究硫转运蛋白的功能及硫酸根运输的机理奠定了基础。[ Objective ] Subcellular location of a sulfate transporter gene OsST in Oryza sativa L. was studied by constructing a fusion expres- sion vector of YFP and OsST. [ Method]The open reading frame of OsST was cloned from rice leafg cDNA by RT-PCR, and ligated into the vector of pAT-YFP after sequencing. Recombinant plasmid was introduced into onion epidermal cells by the particle bombardment method. Yellow fluorescence was monitored under a Laser scanning confocal microscope. [ Result] The OsST protein was located in the plasma mem- brane and nucleusr membrane. [ Conclusion ] The result lays a foundation for studying the function of sulfate transporter and mechnism of transporting SO^24 in rice.

关 键 词：硫转运蛋白 亚细胞定位 黄色荧光蛋白 水稻 

分 类 号：S132[农业科学—农业基础科学]