颠茄高频再生体系的建立及卡那霉素抗性筛选  

Establishment of Atropa belladonna L. High Frequency Regeneration System and the Kanamycin Resistance Screening

作　　者：王细荣[1] 强玮[1] 卢衍[1] 李金弟[1] 陈敏[1] 廖志华[1] 

机构地区：[1]西南大学生命科学学院,重庆400715

出　　处：《安徽农业科学》2011年第17期10197-10198,10373,共3页Journal of Anhui Agricultural Sciences

基　　金：国家"863"计划项目(2010AA100503);重庆市自然科学基金项目(2009-BB5309);教育部中央高校基本业务费(XDJK2010C087);重庆市高等学校优秀人才资助计划项目

摘　　要：[目的]建立颠茄高频再生体系及筛选卡那霉素(Kan)抗性。[方法]以颠茄叶片及腋芽为外植体,研究了培养基中6-BA和NAA不同配比对其不定芽分化的影响以及叶片不定芽对Kan的敏感性。[结果]MS+4.5mg/L6-BA+0.2mg/LNAA为叶片不定芽分化的最佳培养基,不定芽分化率达100%,在1cm×1cm叶块上的不定芽分化数平均达5.85;MS+3.0mg/L6-BA+0.1mg/LNAA为腋芽不定芽分化的最适培养基,不定芽分化率达100%,每个腋芽不定芽分化平均数为4～8个;400.0mg/LKan为颠茄叶片遗传转化的最佳筛选浓度。[结论]为颠茄无菌苗的快速繁殖以及基于叶盘法的遗传转化奠定了基础。[ Objective ] The research aimed to establish the high frequency regeneration system of Atropa belladonna L. and screen the kana- mycin （Kan） resistance. [ Metho.d] The leave and axillary buds of Atropa belladonna L. were the explants. The influence of different ratio of 6-BA and NAA in the medium on the adventitious bud differentiation and the sensibility of leaf adventitious bud on kan were studied. [ Result] MS ＋ 2.5 mg/L 6-BA ＋ 0.2 mg/L NAA was the optimum medium of leaf adventitious bud differentiation, and the differentiation ratio of adven- titious bud reached 100%. The number of adventitious bud differentiation on 1 cm x 1 cm leaf block averagely reached 5.85. MS ＋ 3.0 mg/ L 6-BA ＋ 0.1 mg/L NAA was the optimum medium of axillary bud adventitious bud differentiation, and the differentiation ratio of adventitious bud reached 100%. The average number of adventitious bud differentiation in every axillary bud was during 4 - 8. The optimum kanamycin screening concentration of Atropa belladonna L. leaf genetic transformation was 400.0 mg/L. [ Conclusion] The research laid the foundation for the intermediate propagation of Atropa belladonna L. aseptic seedling and the genetic transformation based on the leaf disc cocultivation.

关 键 词：颠茄 再生体系 Kan 敏感性 

分 类 号：S567[农业科学—中草药栽培]