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作 者:戴菲[1] 黄运红[1] 彭伟梦[1] 李鹿鸣[1] 龙中儿[1]
机构地区:[1]江西师范大学生命科学学院,江西南昌330022
出 处:《安徽农业科学》2011年第17期10202-10203,共2页Journal of Anhui Agricultural Sciences
基 金:江西省自然科学基金项目(2009GQN0068)
摘 要:[目的]研究东乡普通野生稻(Oryza rufipogon Griff.)根际微生物群落特征。[方法]采用氯仿熏蒸浸提法测定根系微生物生物量碳、氮;采用平板稀释培养法测定群落组成;采用最大或然计数法测定微生物生理群。[结果]东乡野生稻根际微生物生物量碳、氮平均值分别为83.02和16.98mg/kg(土),较普通栽培稻低;根际可培养微生物中细菌>放线菌>真菌;氮素生理群中氨化细菌>好氧固氮菌>反硝化细菌>嫌气性固氮菌>硝化细菌>亚硝化细菌。[结论]东乡野生稻根际微生物群落组成与普通栽培稻相比有差异。[ Objective ] The aim was to study the characteristics of microbial community in the rhizosphere soil of Dongxiang wild rice ( Oryza rufipogon Griff. ). [ Method ] The microbial biomass carbon and nitrogen was estimated by the chloroform fumigation extraction method; the microbial commui^ity composition and nitrogen cycling microbial functional groups were investigated by the dilution plate culture method and the most probable~ number methods respectively. [ Result ] The microbial biomass carbon and nitrogen, in rhizosphere soil of Dongxiang wild rice was 83.02 and 16.98 mg/kg( soil), which was lower than that of ordinary cultivated rice ; The relationship between the number of cuhur- able microbial groups was bacteria 〉 actinomycetes 〉 fungi, and the nitrogen cycling microbial physiological groups was as the following: ammonifying bacteria 〉 aerobic azotobacteria 〉 denitrobacteria 〉 anaerobic azotobactefia 〉 nitrobacteria 〉 nitrosobacteria. [Conclusion] The microbial community in the rhizosphere soil of Dongxiang wild rice was different from that of the ordinary cultivated rice.
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