苹果砧木耐盐变异体的生理生化及分子生物学分析  

Physiological-biochemistrical and Molecular Biological Analysis of Salt-tolerant Mutant of Apple Rootstock

作　　者：李振侠[1,2] 徐继忠[1] 邵建柱[1] 

机构地区：[1]河北农业大学园艺学院,河北保定071001 [2]河北科技大学生命科学与工程学院,河北石家庄050018

出　　处：《安徽农业科学》2011年第17期10227-10228,10243,共3页Journal of Anhui Agricultural Sciences

基　　金：河北省科技攻关项目(06220117D-3)

摘　　要：[目的]筛选苹果砧木耐盐变异体,并对其生理生化和分子生物学特征进行分析。[方法]用60Co-γ对苹果砧木78-48进行辐射处理,以处理明显的叶片为外植体诱导出再生植株,以1.0%和1.2%NaCl作为选择压,对再生植株进行多代交替盐筛选,直到筛选出耐1.2%NaCl的变异体L和K,以耐盐变异体L2和K2为试材,对其进行耐盐生理生化指标测定及RAPD分析。[结果]变异体不仅在生理生化指标上有明显的差异,而且在DNA水平上发生了突变。[结论]变异体L2和K2是78-48的耐盐新株系。[ Objective ] The research aimed to screen the salt- tolerant mutant of apple rootstock, and analyze their physiological- biochemis-trical and molecular biological characteristics. [ Method ] The original line 78-48 （CK） was irradiated by ^60Co-y, and the effect obvious leaves were chose to induce the regenerative plants, thenthe plants were alternating salt screened taking 1.0% and 1.2% NaC1 as selection press, until to screen the mutants to 1.2% NaC1- tolerance. Finally, the physiological- biochemistrical indices of the salt- tolerant mutants, L2 and K2, were determined, and their molecular biological characteristics were studied by RAPD analysis. [ Result] The result showed that com- pared with the original line, the mutants varied not only on the salt-tolerant indexes level, but also on the DNA level, which provided a strong evidence for the truth of the mutants. [ Conclusion] These mutants （L2 and K2） were sah-tolerant new lines of apple rootstock.

关 键 词：苹果砧木 耐盐变异体 脯氨酸 质膜透性 RAPD 

分 类 号：S661[农业科学—果树学]