蚕豆根尖细胞微核技术监测实验室有机废水的污染研究  

Study on Organic Wastewater Monitoring of Laboratory Using MCN Test of Vicia Faba Root Tips

作　　者：闫晶晶[1] 屈艾[1] 刘小燕[1] 贾蓉[1] 

机构地区：[1]徐州师范大学生命科学学院,江苏徐州221116

出　　处：《安徽农业科学》2011年第17期10417-10419,10517,共4页Journal of Anhui Agricultural Sciences

摘　　要：[目的]对生物及化学类实验室的有机废水进行遗传毒性和污染效应的研究。[方法]将实验室条件下制备环己酮时所排有机废水逐级稀释成8个梯度浓度,采用蚕豆(Viciafaba)根尖细胞微核技术进行检测。[结果]不同浓度梯度的实验室有机废水对蚕豆根尖细胞有遗传毒性,在一定浓度范围内,随着废水处理浓度增大,蚕豆根尖细胞微核率、污染指数和有丝分裂指数逐渐增加;但浓度过高,反而会抑制蚕豆根尖细胞微核的形成和有丝分裂的进行。[结论]实验室有机废水有明显的遗传毒性和环境污染效应,可以利用蚕豆根尖细胞微核技术对其污染效应进行监测和评价,实验室废水必须进行合理的处理(稀释或吸附消除重金属)方能排出。[ Objective] The genetic toxicity and pollution effects of biological and chemical organic wastewater were studied. [ Method J Or- ganic wastewater which was formed during the preparation of cyclohexanone were diluted eight concentration gradients and detected using MCN test of Vicia faba root tips. [ Results ] The results showed that different concentration gradients of organic wastewater had genetic toxicity. In certain concentration range, with the rising of organic wastewater treatment concentration, rate of micronucleus of Viciafaba root tip ceils, pol- lution index and mitotic index gradually increased. However, beyond concentration gradient, organic wastewater restrained Viciafaba cell mi- cro nuclear formation and mitosis. [ Conclusion ] Organic wastewater has obvious genetic toxicity and environment pollution effect. VAMCN can be used to monitor and evaluate pollution effect of organic wastewater and the laboratory wastewater after reasonably diluted or eliminate heavy metals can be discharged.

关 键 词：实验室有机废水 微核技术 遗传毒性 污染效应 

分 类 号：X703[环境科学与工程—环境工程]