尿中肾损伤标志物检测在儿科疾病诊断中的应用价值  

URINE MARKERS OF RENAL INJURY IN THE DIAGNOSIS OF PEDIATRIC VALUE

作　　者：秦望森[1] 

机构地区：[1]河南省人民医院,郑州450000

出　　处：《现代预防医学》2011年第14期2813-2814,共2页Modern Preventive Medicine

摘　　要：[目的]结合临床实践经验,探讨尿中肾损伤标志物检测在儿科疾病诊断中的应用价值。[方法]采用对照研究,被检儿童均留取新鲜尿液(除晨尿)10ml,-20℃保存待检。试剂、标准品、质控品由上海太阳生物技术公司提供。离心后标本用ELISA法测定IgG、RBP、β2-MG、m-ALB,在酶标仪492nm处读OD值计算结果,并采用比色法测NAG、721分光光度计405nm处比色并计算结果。对比分析两组的检测结果,分析尿中肾损伤标志物检测在儿科疾病诊断中的应用价值。[结果]肾病组尿中标志物水平均显著高于正常对照组,差异有统计学意义(P﹤0.05),肾病组各肾损伤标志物测定水平较高,联合应用100%阳性,而正常对照组仅有5.00%为阳性(主要为IgG水平较高,考虑主要与其出现在非选择性蛋白尿有关),两组比较差异有统计学意义(χ2=21.93,P﹤0.05)。[结论]对于多种原因造成的肾脏损伤,可以检测尿中IgG、RBP、β2-MG、m-ALB、NAG水平,不仅比血中BUN、Cr等肾功能指标敏感,而且实验标本留取简便,有助于临床早期发现肾损伤,积极采取合理用药并判断预后。[Objective]By clinical practice,we explore the application of urinary markers of renal injury in the diagnosis of pediatric value.[Methods]A controlled study,children were urine specimens from fresh（except for morning urine）10 ml,-20℃ saved for inspection.Reagents,standards,control materials from Shanghai Sun Biotech companies.After centrifugation,specimens were determined by ELISA IgG,RBP,β2-MG,m-ALB,read in the microplate reader at OD 492 nm value of the results,and measured by colorimetry NAG,721 colorimetric spectrophotometer at 405 nm and results.Compared two groups of test results,we analyzed the urinary markers of renal injury in the diagnosis of pediatric value.[Results]Urinary markers of nephropathy group were significantly higher than the normal control group,there was significant difference between the two groups（P﹤0.05）.The nephropathy,renal injury markers determined the high level,combined with 100% positive,while the normal control group 5.00% are positive（mainly higher level of IgG,the main consideration in their non-selective proteinuria related）,there was significant difference between the two groups（χ2 = 21.93,P﹤0.05）.[Conclusion]For a variety of causes kidney damage,IgG,RBP,β2-MG,m-ALB and NAG levels can be detected in urine,not only Bibi blood BUN,Cr and other sensitive indicators of renal function,and the experimental sample specimens from simple,early detection of renal injury can help clinicians to actively take rational use of drugs and determine prognosis.

关 键 词：肾损伤标志物检测 肾小管上皮细胞损伤 小儿肾脏疾病 

分 类 号：R72[医药卫生—儿科]