RP-HPLC测定桑椹中芦丁的含量  

Determination of Rutin in Morus alba by RP-HPLC

作　　者：耿旦[1] 马雯芳[2] 甄汉深[2] 丘琴[2] 韦良宏[1] 唐霞丽[2] 

机构地区：[1]钦州市第一人民医院,广西钦州535000 [2]广西中医学院,南宁530001

出　　处：《中国实验方剂学杂志》2011年第14期63-65,共3页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：广西卫生厅项目(Z2009238)

摘　　要：目的:优化桑椹中芦丁的提取条件并建立芦丁的HPLC测定方法。方法:采用单因素考察法优化桑椹中芦丁的提取条件,采用HPLC法测定芦丁的含量,色谱柱为Agilent Zobax C18(4.6 mm×250 mm,5μm),以乙腈-0.1%磷酸(19∶81)为流动相,流速1.0 mL.min-1,检测波长360 nm,柱温30℃。结果:芦丁最佳提取条件为料液比1∶25,50%乙醇回流60 min,芦丁在0.1～2.0μg有良好线性关系(r=0.999 9)。平均回收率为102.4%,RSD 1.8%(n=6)。结论:该方法简便、准确、可靠,可作为桑椹中芦丁含量测定的方法。Objective：Optimize the extract condition of rutin in Morus alba to establish a quantification method for rutin.Method： Single-factor analysis was applied to optimize the extract condition of rutin in Morus alba HPLC was used in the determination of rutin on C18 column（4.6 mm × 250 mm,5 μm） with a mixture of acetonitrile-0.1% phosphoric acid（19：81） as a mobile phase at the flow rate of 1.0 mL.min-1.Rutin was detected at 360 nm,column temperature was 30 ℃.Result： The optimal extract condition was the solid-to-liquid ratio 1：25,50% ethanol refluxed for 60 min.The linearity of rutin was in the range of 0.1-2.0 μg.The average recovery rate of rutin was 102.4% with RSD 1.8%（n = 6）.Conclusion： The determination method was simple,accurate and reliable,it could be used as the determination method of rutin in Morus alba.

关 键 词：反相高效液相色谱法 桑椹 芦丁 含量测定 

分 类 号：R284.1[医药卫生—中药学]