马兜铃酸A在中药饮片及其配方颗粒中的含量分析  

Assaying of Aristolochic Acid A in Chinese Herbal Pieces and the Traditional Chinese Medicine Particle

作　　者：黄国鑫[1] 倪晨[1] 李一圣[1] 易宇阳[1] 张仲敏[1] 赵学军[1] 苏子仁[1] 

机构地区：[1]广州中医药大学中药学院,广州510006

出　　处：《中国实验方剂学杂志》2011年第14期75-78,共4页Chinese Journal of Experimental Traditional Medical Formulae

摘　　要：目的:对5种马兜铃科中药饮片及配方颗粒(由相应批次的中药饮片制成)所含马兜铃酸A进行含量测定。方法:采用高效液相色谱法,YMC-Pack C18色谱柱(4.6 mm×250 mm,5μm),流动相甲醇(A)-0.05%磷酸溶液(B),检测波长250nm,流速0.8 mL.min-1,柱温30℃。结果:5种马兜铃科中药饮片(各3批)中马兜铃酸A平均质量分数分别为关木通4.18mg.g-1,马兜铃0.77 mg.g-1,蜜马兜铃0.46 mg.g-1,青木香0.86 mg.g-1,寻骨风0.53 mg.g-1;5种中药配方颗粒(n=3)马兜铃酸A平均质量分数分别为关木通1.16 mg.g-1,马兜铃0.31 mg.g-1,蜜马兜铃0.10 mg.g-1,青木香0.39 mg.g-1,寻骨风0.03 mg.g-1。结论:建立的方法对多种含马兜铃酸A药材与配方颗粒具有较好的适应性,可以用于其含量测定;中药配方颗粒的制作工艺对中药饮片的成分有一定影响,有必要建立相关生产监控标准、质量控制标准,用来保证中药配方颗粒的质量。Objective：The content of aristolochic acid A in the five kinds of medical materials and dispensing granules of Aristolochiaceae family was determined to provide scientific foundation for promoting development of our traditional Chinese medicine particle.Method： HPLC method was applied on YMC-Pack C18 column（4.6 mm×250 mm,5μm） at 30 ℃ with methanol（A）-0.05% phosphoric acid（B） as the mobile phase;detected wavelength was at 250 nm,and the flow rate was 0.8 mL·min-1.Result： The content of aristolochic acid A in the five kinds of medical materials,i.e.Akebia stem,Dutchmanspipe Fruit,honeyed Dutchmanspipe Fruit,Radix Aristolochiae and Datchmanspipe Herb was 4.18 mg·g-1,0.77 mg·g-1,0.46 mg·g-1,0.86 mg·g-1,0.53 mg·g-1 respectively.The content of aristolochic acid A in the five kinds of dispensing granules was 1.16 mg·g-1 for Akebia stem,0.31 mg·g-1 for Dutchmanspipe Fruit,0.10 mg·g-1 for honeyed Dutchmanspipe Fruit,0.39 mg·g-1 for Radix Aristolochiae and 0.03 mg·g-1 for Datchmanspipe Herb respectively.Conclusion： The method established in this paper has relative adaptability for various kinds of medical materials and dispensing granules containing aristolochic acid A.It can be used for content determination.The production process of traditional Chinese medicine particle prescription has certain effect to composition of medical materials,so it is necessary to establish relevant production monitoring criterion,quality monitoring criterion for ensuring the quality of traditional Chinese medicine particle prescription.

关 键 词：马兜铃酸A 中药饮片 中药配方颗粒 

分 类 号：R284.1[医药卫生—中药学]