舒肝凉血方对MCF-7和T47D细胞增殖及硫酸酯酶活性和表达的影响  

Effects of Shugan Liangxue Decoction on MCF-7 and T47D Cells Proliferation,Enzymatic Activity and mRNA Expression of Steroid Sulfatase

作　　者：付雪松[1] 李萍萍[1] 

机构地区：[1]北京大学临床肿瘤学院、北京肿瘤医院暨北京市肿瘤防治研究所中西医结合科,恶性肿瘤发病机制及转化研究教育部重点实验室,北京100142

出　　处：《中国实验方剂学杂志》2011年第14期144-147,共4页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：国家自然科学基金项目(200930973906)

摘　　要：目的:观察舒肝凉血方对雌激素受体阳性乳腺癌MCF-7和T47D细胞增殖的影响,并检测舒肝凉血方对两个细胞系中硫酸酯酶的活性及其mRNA表达的影响。方法:培养雌激素受体阳性的乳腺癌细胞MCF-7和T47D,给予不同浓度的舒肝凉血方处理24 h后,MTT法检测复方对MCF-7和T47D细胞增殖的影响;RT-PCR检测舒肝凉血方对硫酸酯酶mRNA表达的影响。培养MCF-7和T47D细胞,转染萤火虫荧光素酶和海肾荧光素酶质粒,给予不同浓度的舒肝凉血方处理24 h后,双荧光素酶报告基因检测试剂盒检测复方对两个细胞系中硫酸酯酶活性的影响。结果:舒肝凉血方显著抑制了MCF-7和T47D细胞的增殖,并呈现出剂量依赖关系,半数抑制浓度(IC50)分别是46.08 g.L-1和22.98 g.L-1;低浓度的舒肝凉血方对硫酸酯酶mRNA的表达无明显影响,高浓度时则显著下调了硫酸酯酶mRNA的表达;舒肝凉血方显著降低了MCF-7和T47D细胞中硫酸酯酶的活性,且呈现出剂量依赖性。结论:舒肝凉血方能显著抑制MCF-7和T47D细胞的增殖,此作用可能与该方降低硫酸酯酶活性以及下调硫酸酯酶mRNA的表达有关。Objective：To investigate the effects of Shugan Liangxue decoction（SGLXD） on MCF-7 and T47D cells proliferation,and evaluate the effects of SGLXD on the enzymatic activity and mRNA expression of steroid sulfatase（STS）.Method： Estrogen receptor positive breast cancer MCF-7 and T47D cells were cultured and treated with different concentrations of SGLXD for 24 h.Cell proliferation was evaluated by MTT assay,and the mRNA expression of STS was investigated by RT-PCR.After transient co-transfection with plasmid pERE-TK-Luc and pRL-TK in MCF-7 and T47D cells,and treated with different concentrations of SGLXD for 24 h,we evaluated the enzymatic activity of STS by dual-luciferase reporter assay（DLR）.Result： By MTT assay,SGLXD inhibited the cell proliferation of MCF-7 and T47D dose-dependently,the IC50 values were 46.08 g·L-1 and 22.98 g·L-1.SGLXD down-regulated the expression of STS mRNA at higher concentrations significantly.In MCF-7 and T47D cells,the enzymatic activity of STS was significantly inhibited by SGLXD dose-dependently.Conclusion： SGLXD can inhibit the cell proliferation of MCF-7 and T47D,this effect may be associated with the inhibitory effects of SGLXD on the enzymatic activity of STS and the expression of STS mRNA.

关 键 词：舒肝凉血方 硫酸酯酶 乳腺癌 

分 类 号：R285.5[医药卫生—中药学]