异鼠李素对H_2O_2损伤内皮细胞的保护作用  

Protective Effect of Isorhamnetin on H_2O_2-induced Injury of Human Umbilical Vein Endothelial Cells

作　　者：张放[1] 程嘉艺[1] 苑博[1] 

机构地区：[1]辽宁中医药大学,沈阳110032

出　　处：《中国实验方剂学杂志》2011年第14期169-172,共4页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：辽宁省教育厅2009年度高等学校科研项目计划(2009A495)

摘　　要：目的:观察异鼠李素对H2O2损伤的CRL-1730细胞的活性、凋亡率以及细胞周期的影响。方法:用55,27.5,13.75 mg.L-13种质量浓度的异鼠李素与培养的CRL-1730细胞置于37℃,5%CO2饱和湿度培养箱中共同孵育24 h,再用H2 O2氧化损伤4 h后,四甲基偶氮唑蓝(MTT)比色法检测异鼠李素对H2 O2损伤的细胞活性的影响,流式细胞仪测定异鼠李素对H2O2损伤的细胞周期及细胞凋亡的影响。结果:异鼠李素能够剂量依赖性增强CRL-1730细胞活性,与模型组吸光度(A)0.459比较,高剂量组A 0.503,升高了0.044,有显著性差异(P<0.01),中剂量组A 0.48,升高了0.027,差异有统计学意义(P<0.05),低剂量组无明显影响。异鼠李素能够剂量依赖性减少受损细胞的凋亡,降低早期凋亡率,与模型组凋亡率77.78%相比,高剂量组69.28%和中剂量组72.50%差异均有统计学意义(P<0.05)。异鼠李素能抑制H2 O2引起的CRL-1730细胞减少,表现在使G0/G1期细胞比例减少,模型组G0/G1期细胞比例为82.23%,异鼠李素高,中,低剂量组该值分别为69.43%,67.05%,69.56%,均有显著性差异;S期和C2/M期细胞比率增加,模型组S期和C2/M期细胞比率为11.77%和1.91%,异鼠李素高剂量组为23.39%和7.18%,中剂量组为29.73%和3.23%,低剂量组为27.42%和3.01%。差异均有统计学意义(P<0.05或P<0.01)。结论:异鼠李素对H2O2损伤的CRL-1730细胞具有保护作用。Objective：To observe the influence of isorhamnetin on cell cycle and cell apoptosis in H2O2-induced injury of human umbilical vein endothelial cells（HUVECs）.Method：Cultured CRL-1730 cell was commonly incubated at 37 ℃ in 5%CO2 for 24 hours with isorhamnetin of different concentrations,then CRL-1730 was injured by H2O2 for 4 hours.Cell viability was measured by MTT assay.The rate of apoptosis and cell circles were detected by flow cytometry（FCM）.Result： Iisorhamnetin could increase the cell viability in a concentration-dependent manner.Compared with the model group,the high dosage group showed A of 0.503（increased 0.044）,with significant difference（P0.01）.Low dosage group was no significant difference,while the middle dosage group showed A of 0.48（increased 0.027）,with significance（P0.05）.Isorhamnetin could decrease the cell apoptosis in a concentration-dependent manner,especially decrease the early apoptosis.Compared with the model group apoptosis rate of 77.78%,the apoptosis in high dosage group was 69.28% and the middle dosage group was 72.50%.Both had statistical significance（P0.05）.Isorhamnetin could inhibit the decrease in CRL-1730 caused by H2O2,showing by the cell cycle ratio of G0/G1.In the model group the ratio was 82.23%,in the high,middle and low dosage groups of isorhamnetin they were 69.43%,67.05% and 69.56% accordingly.All of them had significance.Isorhamnetin increased the percentage of cells in S phase and C2/M phase,the percentage of cells of S phase and C2/M phase of the model group was 11.77% and 1.91% respectively,while the high dosage was 23.39% and 7.18%,the middle dosage was 29.73% and 3.23%,and the low dosage was 27.42% and 3.01%.Conclusion： Isorhamnetin has protective effect on HUVECs injury induced by H2O2.The mechanism may be related to its antioxidant activity.

关 键 词：异鼠李素 过氧化氢 CRL-1730 细胞周期 凋亡率 

分 类 号：R285.5[医药卫生—中药学]