附子对脾阳虚小鼠的抗寒泻作用  

Anti-cold Diarrhea Effect of Aconiti Lateralis Radix Praeparata on Mice with Spleen Yang Deficiency Syndrome

作　　者：邵峰[1] 李赛雷[1] 刘荣华[1] 黄慧莲[1] 任刚[1] 林红军[1] 

机构地区：[1]江西中医学院现代中药制剂教育部重点实验室,南昌330004

出　　处：《中国实验方剂学杂志》2011年第14期176-178,共3页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：国家自然科学基金项目(30801520);国家中医药管理局中医药行业科研专项中药类项目(200807020);江西省教育厅项目(GJJ10212)

摘　　要：目的:探讨附子对脾阳虚小鼠的抗寒泻作用。方法:采用苦寒泻下法进行造模,将小鼠连续ig番泻叶水煎液29d,复制脾阳虚小鼠模型。再以附子混悬液(0.42,0.84,1.68 g.kg-1)ig治疗10 d。采用炭末推进法、耐寒以及耐缺氧实验,分别以小肠推进率、-10℃低温条件下和常压缺氧条件下的存活时间为考察指标,比较附子不同给药剂量对脾阳虚小鼠小肠推进、给药前后小鼠体重、耐寒能力以及耐缺氧能力的影响。结果:附子(0.84,1.68 g.kg-1)对脾阳虚小鼠小肠推进运动均具有明显的抑制作用(P<0.05),而且能使小鼠体重明显上升(P<0.05,P<0.01);附子高剂量(1.68 g.kg-1)能明显延长脾阳虚小鼠在-10℃低温条件下和常压缺氧条件下的存活时间(P<0.05)。结论:附子能使脾阳虚小鼠模型恢复趋于正常,提示附子对脾阳虚小鼠具有良好的抗寒泻作用。Objective：To observe anti-cold diarrhea effect of Aconiti Lateralis Radix Praeparata on mice with spleen Yang deficiency syndrome.Method： Mice were fed with decoction of Sennae Folium for 29 days to induce the model of spleen Yang deficiency syndrome.After modeling,Aconiti Lateralis Radix Praeparata suspension was given（in the doses of 0.42,0.84,1.68 g·kg-1） for 10 days.After treatment,small intestinal propulsion rate（by charcoal powder propelling experiment）,survival time under-10 ℃（by cold tolerance experiment） and anoxia condition（anoxia tolerance experiment） were recorded,respectively.The effects of three dosages of Aconiti Lateralis Rradix Praeparata suspension on small intestinal propulsion,cold tolerance and anoxia tolerance were investigated.Result： After administration of Aconiti Lateralis Radix Praeparata（0.84,1.68 g·kg-1）,an inhibition of small intestinal propulsion（P0.05） and body weight-increasing（P0.01） in mice with spleen Yang deficiency were observed when compared with the model group.The survival time under-10 ℃（P0.05） and anoxia condition（P0.05） were pronouncedly prolonged by the administrating with Aconiti Lateralis Rradix Praeparata at the dose of 1.68 g·kg-1.Conclusion：Aconiti Lateralis Radix Praeparata has an obvious therapeutic efficacy on mice with spleen Yang deficiency syndrome.

关 键 词：附子 脾阳虚 小肠推进 耐寒 耐缺氧 

分 类 号：R285.5[医药卫生—中药学]