开郁胶囊对抑郁动物模型的影响  

Effects of Kaiyu Capsule on Depression-like Animal Models

作　　者：李雅莉[1] 赵玲[1] 魏海峰[1] 李林[1] 

机构地区：[1]首都医科大学宣武医院药物研究室,神经变性病教育部重点实验室,北京100053

出　　处：《中国实验方剂学杂志》2011年第14期182-185,共4页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：北京市新医药学科群建设项目(XK100270569)

摘　　要：目的:开郁胶囊是本室自行研制的由8种传统中药组成的中药新复方制剂,本研究观察开郁胶囊对数种抑郁动物模型的影响。方法:采用ICR小鼠随机分为对照组、开郁胶囊低、中、高剂量组(按生药量计为6.6,13.2,26.4 g.kg-1)及阳性对照药帕罗西汀组。连续ig 14 d,于末次给药1.5 h后采用小鼠悬尾、强迫游泳、5-羟色胺酸(5-HTP)诱导甩头及利血平拮抗实验动物模型,评价开郁胶囊的抗抑郁作用。结果:与对照组相比,开郁胶囊中、高剂量组能显著缩短小鼠悬尾不动时间(P<0.05);开郁胶囊高剂量组显著增加5-HTP诱导的小鼠甩头次数(P<0.01),缩短甩头潜伏期(P<0.05)。注射利血平后动物出现利血平化,即眼睑下垂、体温下降及活动抑制;开郁胶囊中、高剂量ig 14 d,可改善利血平化小鼠的眼睑下垂及活动抑制(P<0.01),对体温下降亦有显著的拮抗作用(P<0.01)。结论:开郁胶囊对拟抑郁症小鼠模型具有明显的拮抗作用,其作用机制可能与增强5-羟色胺能和去甲肾上腺素能神经功能有关。Objective：Kaiyu capsule is a new compound preparation which consists of 8 traditional Chinese herbs.The purpose of the study was to investigate the effects of Kaiyu capsule on depression-like animal models.Method： ICR mice were randomly allocated to five groups： control,Kaiyu capsule low,middle and high dose groups（6.6,13.2,26.4 g·kg-1 accordingly）,and Paroxetine（positive control）.After intragastric administration for 14 d,the antidepressant effect of Kaiyu capsule was evaluated by using mice tail suspension test,mice forced swimming test,5-hydroxytryptophan（5-HTP）-induced head-twitches and reserpine reversal model in mice.Result： Compared with control group,the middle and high doses of Kaiyu capsule significantly decreased the accumulative immobility time in mice tail suspension test（P0.05）.High dose Kaiyu capsule obviously increased the head-twitching number induced by 5-HTP（P0.01）,and shortened the latency period（P0.05）.After sc reserpine,the mice appeared physical signs such as blepharoptosis,akinesia and hypothermia.The middle and high doses of Kaiyu capsule significantly ameliorated the blepharoptosis and akinesia（P0.01）,and decreased the range of hypothermia in reserpine-induced model mice（P0.01）.Conclusion： Kaiyu capsule has the significant antidepressant effect,and the mechanisms may be related to the enhancement of serotonergic and noradrenergic functions.

关 键 词：抑郁症 悬尾 强迫游泳 利血平 5-羟色胺 去甲肾上腺素 开郁胶囊 

分 类 号：R285.5[医药卫生—中药学]