毕岱总皂苷对实验性高血脂症大鼠血脂代谢的影响  

Effects of Total Saponins of Medicago sativa on Blood Lipid Metabolism in Rats with Experimental Hyperlipidemia

作　　者：刘斐[1] 陈虹[1] 钟明[1] 魏丽丽[1] 刘春丽[1] 刘春阳[1] 

机构地区：[1]石河子大学药学院,教育部新疆特种植物药重点实验室,新疆石河子832002

出　　处：《中国实验方剂学杂志》2011年第14期211-214,共4页Chinese Journal of Experimental Traditional Medical Formulae

摘　　要：目的:建立高血脂症大鼠模型,观察毕岱总皂苷(BD-TAS)对高血脂症大鼠血脂代谢的影响。方法:健康成年雄性SD大鼠60只,随机分为正常对照组(NC),高脂模型组(HC),血脂康对照组,毕岱总皂苷组(BD-TAS,低、中、高剂量组);除NC组,其余各组高脂饲料喂养8周,建立高脂血症大鼠模型;从模型复制成功后第0周开始,NC组和HC组均ig等量生理盐水,BD-TAS低、中、高剂量组(4,9,12 g.kg-1)和血脂康对照组(0.5 g.kg-1)均ig给药,并在第0,2,4,6周后称量体重,眼眶取血,离心取血清,用酶法测定血浆中胆固醇(TC)、甘油三酯(TG)和高密度脂蛋白胆固醇(HDL-C)的水平以及血清和肝脏丙二醛(MDA)水平,评价BD-TAS对高血脂症大鼠血脂代谢和肝脏保护作用。结果:以高脂饲料喂养大鼠8周后,与NC组相比,HC组TC,TG,HDL-C和LDL-C均升高且有显著性差异(P<0.01),成功复制了混合型高脂血症大鼠模型。以不同剂量BD-TAS溶液ig后,各组血浆TC,TG水平与高脂模型组比较,在第2,4,6周具有显著性差异(P<0.05或P<0.01);与NC组和HC比较,BD-TAS低、中剂量组TC,TG水平降低明显(P<0.05);BD-TAS高剂量组与低剂量组比较,TC,TG水平具有显著性差异(P<0.01)。与NC组比较,BD-TAS给药组血清MDA差异显著(P<0.05)。结论:BD-TAS摄入量越大,其改善血脂水平的效果越好,显示出明显的剂量依赖关系,提示毕岱总皂苷具有降血脂的作用,而且具有一定的肝保护作用。Objective：To establish hyperlipidemia model of rats,to observe total saponins of Medicago sative（BD-TAS） on blood lipid metabolism in hyperlipidemia rats.Method： Sixty SD rats were randomly divided into six groups： normal control group（NC）,high-lipid model group（HC）,Xuezhikang capsule group,and BD-TAS group.All groups were induced with high fat diet for 8 weeks except NC,to establish hyperlipemia.NC and HC group were ig physiological saline,BD-TAS（4,9,12 g·kg-1）,Xuezhikang Capsule group（0.5 g·kg-1）.The contents of TC,TG,LDL-C,HDL-C and MDA were examined four times in the plasma after 0,2,4,6 weeks.Result： The result showed that with high fat diet after 8 weeks,compared with NC group,TC,TG,HDL-C and LDL-C were increased significantly（P 0.05）,so hyperlipidemia rat model was established successfully.BD-TAS of different dose groups,TC and TG in plasma were reduced after 2,4,6 weeks,compared with HC group（P0.05）.The level of TC in low-dose BD-TAS group was statistically significant compared with Xuezhikang group（P0.05） and the levels of TC,TG in high-dose BD-TAS group were statistically significant compared with low-dose BD-TAS group（P0.05）.Conclusion： The levels of TC,TG and MDA decreased with the increased of BD-TAS dose,indicating a clear dose-effect relationship.BD-TAS is effective in modulating the blood lipid levels.

关 键 词：毕岱 总皂苷 高血脂症 血脂代谢 

分 类 号：R285.5[医药卫生—中药学]