黑布药膏对兔耳增生性瘢痕胶原合成与降解的影响  

Effect of Heibu Ointment on Synthetise and Degradation of Collagen in Hypertrophic Scar of Rabbit Ear

作　　者：赵丽[1,2] 周晓宏 关洪全[1] 

机构地区：[1]辽宁中医药大学,沈阳110032 [2]辽宁卫生职业技术学院,沈阳110101

出　　处：《中国实验方剂学杂志》2011年第14期215-218,共4页Chinese Journal of Experimental Traditional Medical Formulae

摘　　要：目的:研究黑布药膏对兔耳增生性瘢痕Ⅰ,Ⅲ胶原及基质金属蛋白酶-1 mRAN表达的影响。方法:成年大耳白兔24只,随机分为正常对照组、瘢痕模型组和黑布药膏治疗组。瘢痕模型组和黑布药膏治疗组建立兔耳腹侧面增生性瘢痕动物模型,21 d后,造模成功。在黑布药膏治疗组瘢痕局部和正常对照组对应皮肤涂抹黑布药膏,每3 d 1次,每次1 g,连续用药56d。在用药后第2,4,6,8周分别切取两组瘢痕组织,通过实时定量PCR(RT-PCR)法检测Ⅰ,Ⅲ胶原mRAN及基质金属蛋白酶-1 mRAN的表达。结果:瘢痕模型组与正常对照组比较Ⅰ胶原mRAN表达显著增强,Ⅲ胶原mRAN及基质金属蛋白酶-1 mRAN表达显著减弱(P<0.01)。黑布药膏治疗组在用药后第2,8周Ⅰ胶原mRAN表达分别为(3.62±1.70),(1.34±0.52);模型对照组第2,8周Ⅰ胶原mRAN表达分别为(3.15±1.24),(4.53±1.71),黑布药膏治疗组较模型组显著减弱(P<0.01);黑布药膏治疗组在用药后第2,8周Ⅲ胶原mRAN表达分别为(1.46±0.34),(2.59±0.35),模型对照组Ⅲ胶原mRAN表达分别为(0.88±0.14),(0.37±0.08),黑布药膏治疗组较模型组显著增强(P<0.01);黑布药膏治疗组在用药后第2,8周基质金属蛋白酶-1 mRAN表达分别为(1.77±0.40),(4.76±0.77),模型对照组第2,8周基质金属蛋白酶-1 mRAN表达分别为(0.53±0.14),(0.34±0.04),黑布药膏治疗组较模型组显著增强(P<0.01)。结论:黑布药膏可以通过抑制I型胶原mRNA的表达,促进Ⅲ型胶原、基质金属蛋白酶-1mRNA的表达,增加胶原酶含量,降低瘢痕组织胶原含量,从而抑制兔耳增生性瘢痕组织增生,这可能是黑布药膏抑制兔耳增生性瘢痕增生的机制之一。Objective：To study the effect of Heibu ointment on the expression of type I and type Ⅲ collagen and MMP-1 mRAN in the hypetrrophic scarof rabbit ear.Method： Twenty-four white rabbits were randomly divided into the normal control group,Heibu ointment treatment group and model group.Heibu ointment treatment group and model group were used to establish the models of hypertrophic scar in ears.After twenty-one days,Heibu ointment（1 g） was plastered on the hypetrrophic scar in Heibu ointment treatment group and on the skin in the normal control group once every three days for 56 days.The tissure material was respectively taken on the second,the fourth,the sixth and the eighth week,the expression of type I and type Ⅲ collagen and MMP-1 mRAN were detected by real time PCR（RT-PCR） technique.Result： Compare with the model group,the expression of type I collagen mRAN was obviously weaker（P0.01）,the expression of type Ⅲ collagen and MMP-1 mRAN was increased in the Heibu ointment treatment group（P0.01）.Conclusion： Heibu ointment could inhibit the expression of type I collagen mRAN and promote the expression of type Ⅲ collagen and MMP-1 mRAN,thus it could inhibit the proliferation of hypertrophic scar of the rabbit ear.This maybe one of the mechanisms that the Heibu ointment inhibit the proliferation of hypertrophic scar of rabbit ear.

关 键 词：黑布药膏 兔耳增生性瘢痕 Ⅰ Ⅲ胶原 基质金属蛋白酶-1 

分 类 号：R285.5[医药卫生—中药学]