马齿苋乙醇提取物对D-半乳糖致衰老小鼠学习记忆能力的影响  

Effects of Ethanol Extracts from Portulacae Herba on Learning and Memory Performances in Senile Mice Induced By D-gal

作　　者：张良和[1] 张红英[1] 孙晓宇[1] 

机构地区：[1]延边大学医学部,吉林延吉133002

出　　处：《中国实验方剂学杂志》2011年第14期229-232,共4页Chinese Journal of Experimental Traditional Medical Formulae

摘　　要：目的:研究马齿苋乙醇提取物对D-半乳糖(D-gal)致衰老小鼠的学习记忆能力,并探讨其作用机制。方法:取小鼠随机分为正常组、模型组、马齿苋乙醇提取物(1.0,0.5 g.kg-1)组。除正常组颈背部sc生理盐水外,其他各组均sc D-gal(1g.kg-1),每d 1次,连续给药和造模42 d。用避暗法、水迷宫法和Morris水迷宫法测定衰老小鼠学习记忆能力,同时测定小鼠脑组织丙二醛(MDA)含量、超氧化物歧化酶(SOD)和谷胱甘肽过氧化物酶(GSH-Px)活性。结果:马齿苋乙醇提取物1.0,0.5g.kg-1的潜伏期和错误次数为(174.14±1,85,103.80±3.93)s,(0.11±0.33,0.63±1.41)次,与模型组相比可使衰老小鼠第1次进入暗室的潜伏期明显延长和错误次数明显减少;到达时间和错误次数为(48.45±38.35,58.25±28.41)s,(8.50±4.97,11.50±5.68)次,与模型组相比可使衰老小鼠到达时间缩短和错误次数明显减少;逃避潜伏期和1 min站台穿越次数为(20.33±20.20)s,(3.22±2.86)次,与模型组相比马齿苋乙醇提取物(1 g.kg-1)组可使衰老小鼠逃避潜伏期明显缩短和1min站台穿越次数明显增多;同时明显降低衰老小鼠脑组织MDA含量。结论:马齿苋乙醇提取物可提高D-gal致衰老小鼠学习记忆能力,其机制可能与降低MDA含量有关。Objective：To study the effects of ethanol extracts from Portulacae Herba on learning and memory performances in senile mice induced by D-gal,and its mechanisms of effects.Method： The mice were randomly divided into 4 groups：control group,model group and the ethanol extracts from Portulacae Herba（1.0,0.5 g·kg-1） groups.The control group was given sc of isotonic NaCl in the cervical back part while the other all were given sc D-gal（1 g·kg-1）,once daily.The model was continuously established for 42 d,meanwhile medicine was given.Learning and memory performances were tested with step-through,water maze and Morris water maze test,and to determine the contents of malonaldehyde（MDA） and superoxide dismutase（SOD）,and to determine the activity of glutathione peroxidase（GSH-Px） in brain tissue simultaneously.Result： Compared with the model mice,the ethanol extract from Portulacae Herbacould significantly prolong the latency,and reduced the error frequency.It（1 g· kg-1） could obviously shorten escape latency and pass frequency of 1 min erect position in the senile mice.It could obviously reduce the content of MDA in the senile mice.Conclusion： The ethanol extract from Portulacae Herbacan improves the learning and memory performances of senile mice,the mechanism may be related to decrease in content of MDA in brain tissue.

关 键 词：马齿苋 衰老 学习记忆 超氧化物歧化酶 丙二醛 谷胱甘肽过氧化物酶 小鼠 

分 类 号：R285.5[医药卫生—中药学]