RP-HPLC同时测定止痛方提取物中6个成分的含量  

RP-HPLC simultaneous determination of six active components inextracts of Zhitong formula

作　　者：郑琴[1] 郝伟伟[1] 杨明[1] 王木生[1] 薛鑫[1] 

机构地区：[1]江西中医学院现代中药制剂教育部重点实验室,南昌330004

出　　处：《药物分析杂志》2011年第7期1269-1272,共4页Chinese Journal of Pharmaceutical Analysis

基　　金：江西省教育厅科学技术研究项目(GJJ08333);江西中医学院创新人才实验区招标课题(JZ0902)

摘　　要：目的:采用高效液相色谱法同时测定止痛方提取物中芍药苷、原阿片碱、新乌头碱、延胡索乙素、乌头碱、次乌头碱6个成分的含量。方法:采用Venusil XBP-C18(4.6 mm×250 mm,5μm)色谱柱,流动相为甲醇-乙腈-0.1%氨水(磷酸调pH至9.0)梯度洗脱,流速1.0 mL.min-1,检测波长为230 nm(0～12 min,检测芍药苷)、280 nm(12～26 min,检测原阿片碱)、235 nm(26～29 min,检测新乌头碱)、280 nm(29～31 min,检测延胡索乙素)、235 nm(31～50 min,检测乌头碱、次乌头碱),柱温30℃。结果:6个成分的峰面积与浓度的线性关系良好(r>0.9994);加样回收率为96.7%～102.6%。结论:该方法简便、准确,重复性好,为止痛方制剂的质量控制提供依据。Objective：To develop an HPLC method for the determination of paeoniflorin,protopine,mesaconitine,tetrahydropalmatine,aconitine and hypaconitine in extracts of Zhitong formula.Methods：The HPLC analysis was performed on Venusil XBP reversed-phase C18（4.6 mm×250 mm,5 μm） column;The mobile phase consisted of methanol,acetonitrile and 0.1% ammoniae aqua（pH was adjusted to 9.0 by phosphoric acid） with a gradient elution at the flow rate of 1.0 mL·min-1;The optimum detection wavelength was set at three wavelengths in different time period（0-12 min,230 nm for paeoniflorin,12-26 min,280 nm for protopine,26-29 min,235 nm for mesaconitine,29-31 min,280 nm for tetrahydropalmatine,31-50 min,235 nm for aconitine and hypaconitine）,and the column temperature was 30 ℃.Results：Six bioactive components were separated completely in 35 minutes,and had good linear relationship（r0.9994） and recovery（96.7%-102.6%） in their respective detection scope.Conclusion：The method is simple,accurate,and can be used for the quality control of Zhitong preparation.

关 键 词：中成药 芍药苷 原阿片碱 延胡索乙素 乌头类生物碱 高效液相色谱法 

分 类 号：R917[医药卫生—药物分析学]