SPE-UPLC法测定养血清脑颗粒中的芍药苷含量  

SPE-UPLC determination of peoniflorin in Yangxue Qingnao granula

作　　者：李文博[1,2] 韩建平[2] 倪倩[1,3] 高钧[2] 

机构地区：[1]中国药科大学药物分析教研室,南京210009 [2]天津天士力集团有限公司,天津300402 [3]中国药科大学中药分析教研室,南京210009

出　　处：《药物分析杂志》2011年第7期1385-1388,共4页Chinese Journal of Pharmaceutical Analysis

摘　　要：目的:采用SPE-UPLC法分析养血清脑颗粒中芍药苷的含量。方法:采用C18固相萃取柱去除养血清脑颗粒中的基质,超高效液相色谱法测定芍药苷含量。采用Acquity BEHC18(50 mm×2.1 mm,1.7μm)色谱柱,乙腈-水(25∶75)为流动相,检测波长为230 nm,流速为0.2 mL.min-1,柱温40℃。结果:SPE-UPLC法测得芍药苷浓度在5.0～80.0μg.mL-1范围内与其峰面积线性关系良好(r=0.9999),平均回收率(n=6)为99.4%(RSD=2.83%)。结论:该方法准确、简便并能有效去除药物基质保护色谱柱,可用于该制剂的质量控制。Objective：To establish a SPE-UPLC method for the determination of peoniflorin in Yangxue Qingnao granula.Methods：The sample was purified with C18 solid phase column and its content was determined with UPLC method.The UPLC column used in the experiment was C18（50 mm×2.1 mm,1.7 μm）,and the mobile phase was acetonitrile-water（25∶75）at a flow rate of 1.0 mL·min-1;The detection wavelength was 230 nm,and the experiment was accomplished at 40 ℃.Results：The content of peoniflorin determined by SPE-UPLC method showed good linear relationship with the peak areas when their concentrations at 5.0-80·0 μg·mL-1（r=0.9999）,the average recovery（n=6）of peoniflorin was 99.4%（RSD=2.83%）.Conclusion：The method is simple,accurate,effective to reduce the damage of base material to the chromatographic column and can be used to control the quality of Yangxue Qingnao granula.

关 键 词：固相萃取 超高效液相色谱法 中成药 养血清脑颗粒 芍药苷 

分 类 号：R917[医药卫生—药物分析学]