非扫描式光学SPR生物传感器的数据分析  

Data analyses of a non-scanning optical biosensor based on surface plasmon resonance

作　　者：赵媛媛[1] 肖龙[2] 朱莺[3] 胡建东[1] 江敏[4] 胡枫江[1] 魏文松[1] 李会芹[1] 

机构地区：[1]河南农业大学机电工程学院,河南郑州450002 [2]河南职业技术学院,河南郑州450046 [3]浙江省标准化研究院,浙江杭州310006 [4]河南农业大学生命科学学院,河南郑州450002

出　　处：《河南农业大学学报》2011年第3期323-327,共5页Journal of Henan Agricultural University

基　　金：河南省教育厅创新人才项目(2009HASTIT019);河南省杰出青年基金项目(104100510017);郑州市科技领军人才项目(096SYJH25101)

摘　　要：提出了一种用于求解非扫描式光学SPR共振反射光谱信号的共振像素位点的最小二乘法多项式拟合方法,最大限度地提高信噪比.描述了滑动平均值滤波、多项式数据拟合方法的原理和共振反射光谱最低点的质心算法.采用体积分数为13%,14%,16%,17%,18%,19%的乙醇溶液进行试验验证.结果表明,多项式拟合算法对光功率变化等环境因素所引起的噪声具有很好的抑制作用,相关系数为-0.985 2,灵敏度为959.60.This paper proposes a novel design method of optical surface plasmon resonance biosensor in which a laser linear generator was used as a light source of the bioanalyzer,suggesting under appropriate assumptions,an optimal analysis fitting method derived for measuring biomolecular interaction parameters,and a centroid arithmetic for calculating the resonance dip position of a reflected spectra for an decrease in signal to noise ratio.A series of experiments were designed to validate the performance of the optical biosensor using different volume fraction ethanol.In a certain range of volume fraction ethanol,we process the data by polynomial fit and conclude that there is a linear relationchip between the concentration of ethanol and optical response dip of optical SPR.The correlation coefficient is-0.985 2 and sensitivity is 959.60（resonance pixels position per volume fraction ethanol）,suggesting that the fitting algorithm is insensitive to optical power and surrounded factors correlated noise/drift.

关 键 词：表面等离子共振 生物传感器 非扫描 多项式拟合 质心法 

分 类 号：TB133[机械工程—光学工程]