中越金柑种质资源的ISSR分析  

Genetic relationship analysis of Fortunella germplasm resources from China and Vietnam by ISSR markers

作　　者：黄桂香[1] 郭丽英[1] 张树伟[1] 何新华[1] 周瑞阳[1] 陈虎[1] 杨春奖[1] 

机构地区：[1]广西大学农学院,南宁530004

出　　处：《果树学报》2011年第4期563-567,共5页Journal of Fruit Science

基　　金：广西自然科学基金项目(编号:桂科自0991040);科技部国际合作项目(编号:2008DFA30900);广西研究生创新项目(编号:2008105930901D21)

摘　　要：为了更好地利用和创新金柑种质资源,用ISSR分子标记技术分析了25份来自中越金柑属种质资源与金弹变异系的亲缘关系及特异性。从100条引物中筛选出10条多态性好、扩增清晰的引物。结果显示,10条引物共扩增出120条带,其中102条具有多态性,多态性带比率为85%。用UPGMA软件进行聚类分析,25份金柑种质资源的遗传相似系数在0.48～0.99,在遗传系数为0.85水平处将25份供试资源划分为8类,对聚类结果进行分析。中越边境野生山桔资源可能为金柑属的新种类或变种。In order to make good use and innovate germplasm resources of kumquat,the genetic relationships of 25 Fortunella Swingle germplasm resources from China and Vietnam and the variation accessions of F.crassifolia Swingle were examined by ISSR markers in this study.Of the 100 ISSR primers screened,only 10 primers gave reproducible,polymorphic DNA amplification patterns with a total of 120 amplified bands.The percentage of polymorphic band was 85%.By banding patterns obtained from these 10 primers,each accession could be distinguished from the others.The genetic similarity ranged from 0.48 to 0.99 UPGMA relationship tree was established based on the similarity coefficients.At the coefficient level of 0.85,the 25 germplasm resources were divided into 8 groups.The classification results were discussed in this paper.Shanju（Fortunella Swingle） found along the border between China and Vietnam could be a new variety or species of Fortunella Swingle.

关 键 词：金柑 种质资源 ISSR 亲缘关系 金弹变异系 

分 类 号：S666.1[农业科学—果树学]