11个延边地区栽培梨品种的S基因型鉴定  

Determination of S-genotypes of eleven pear cultivars growing in Yanbian region

作　　者：巩艳明[1] 宗成文[1] 曹后男[1] 金灿[1] 李文剑[1] 

机构地区：[1]延边大学农学院,吉林延吉133002

出　　处：《果树学报》2011年第4期575-579,共5页Journal of Fruit Science

基　　金：国家自然科学基金(编号:30960231);延边大学研究生科研项目(延大研科合字2010-35)

摘　　要：鉴定梨品种的S基因型可为合理配置授粉树、杂交亲本选配提供理论依据。为鉴定11个延边地区栽培梨品种的S基因型,利用梨的自交不亲和基因(S-RNase)特异引物FTQQYQ和anti-IIWPNV,对11个梨品种的基因组DNA进行S基因特异扩增,并对扩增片段进行回收、克隆、测序,使用生物信息学软件对各序列进行分析和同源性搜索比对,最终确定各品种的S基因型。结果分别是:苹果梨、东宁五号大梨、延光梨均为S1S17;苹博香为S1S8m;早酥为S1Sd;延边谢花甜为S17S31;尖把梨为S12S30;延边明月梨为S3Se;小香水芽变为SeSx;朝鲜洋梨为SeS3;身不知为S5Sd。Classifying pear S-genotypes is useful for breeding programs and helpful to the selection of suitable pollinators.Using a pair of primers specific to pear S-RNases,PCR amplification of S genes from genomic DNA of eleven Yanbian pear cultivars was carried out.Then the PCR products were extracted,cloned and sequenced.The amplified fragments were assigned to their respective S-RNases by Blast analyses.The S-genotypes of the eleven tested pear cultivars were identified as follows：Pingguoli（S1S17）;Dongning wuhaodali（S1S17）;Yanguangli（S1S17）;Pingboxiang（S1S8m）;Zaosu（S1Sd）;Yanbianxiehuatian（S17S31）;Jianbali（S12S30）;Yanbianmingyueli（S3Se）;Xiaoxiangshuiyabian（SeSx）;Chaoxianyangli（SeS3）;Mishirazu（S5Sd）.

关 键 词：梨 自交不亲和性 DNA序列分析 S基因型鉴定 

分 类 号：S661.2[农业科学—果树学]