^(60)Co γ射线辐照黄连木的生物学效应  

The biological effect of Pistacia chinensis Bunge by ^(60)Co γ-ray irradiation

作　　者：安倩[1,2] 张萍萍[2] 李明浩[2] 陈于和[1] 吴丽芳[2] 

机构地区：[1]河北科技师范学院,秦皇岛063000 [2]中国科学院离子束生物工程学重点实验室,合肥230031

出　　处：《核技术》2011年第7期522-528,共7页Nuclear Techniques

基　　金：国家自然科学基金面上项目(11075178);中国科学院知识创新工程重要方向性项目(KJCX2-YW-N47)资助

摘　　要：用60Coγ射线对黄连木种子进行100、200、400、600、800Gy处理,研究其生物学效应。结果表明,处理后黄连木种子萌发率、成活率和幼苗苗高随剂量增加而下降。200Gy的剂量对黄连木诱变效果较好,成活率和变异率最高。对照相比,辐照处理黄连木幼苗与出现矮化、株型不对称、叶脉失绿、叶片卷曲、畸形等表型变异。用随机引物扩增DNA技术分析(RAPD,Random Amplified Polymorphic DNA)对辐照处理黄连木植株,检测到PCR(Polymerase Chain Reaction)条带的增加和缺失,且条带变异率与剂量呈正相关,而对照植株未发现明显条带变化。Pistacia chinensis Bung seeds were irradiated to 100,200,400,600 and 800 Gy by 60Co γ-rays to study the biological effects.The results show that germination rate and survival rate of Pistacia chinensis seed decreased with increasing doses.The dose 200 Gy group has the highest variation rate and germination rate.The height of M1 seedling decreased with increasing doses.Compared with the control,dwarfing,plant type asymmetry,vein chlorosis,leaf curl,malformation and other phenotypic variation appeared with the irradiation groups of 100-400 kGy.Using RAPD,the M1 plants were investigated.Remarkable variations in the experimental plants were detected by RAPD PCR,but no variation was detected in the control plants.And the 200 kGy group has high frequencies of RAPD variation than the 100 kGy group.

关 键 词：60Co Γ射线 辐照 黄连木 RAPD(Random Amplified POLYMORPHIC DNA) 

分 类 号：Q691[生物学—生物物理学]