羊布鲁氏菌甲酰基转移酶的生物信息学研究  

Bioinformatics Analysis of Formyltransferase of Brucella melitensis

作　　者：王景龙[1,2] 王兴龙[2,3] 

机构地区：[1]吉林大学农学部畜牧兽医学院,吉林长春130062 [2]中国人民解放军军事医学科学院军事兽医研究所,吉林长春130062 [3]吉林省人兽共患病控制与预防中心,吉林长春130062

出　　处：《中国畜牧兽医》2011年第7期123-126,共4页China Animal Husbandry & Veterinary Medicine

基　　金：国家自然科学基金(30972198/C180503);国家转基因新品种培育重大项目(2009ZX08009-163B)

摘　　要：本研究旨在分析和预测羊布鲁氏菌甲酰基转移酶基因及其编码蛋白的结构与功能,用于指导其生物学功能的实验室研究。综合利用生物信息学软件(DNAMAN和Vector NTI)、数据库(prosite和PDB)和网络服务器对甲酰基转移酶进行基本性质分析、三维结构预测和功能预测,展示了甲酰基转移酶的氨基酸分布情况,二级结构中形成螺旋、折叠、卷曲的氨基酸区段及结构域;预测并综合阐释了B细胞表位的柔韧性、表面可及性和亲水性参数。生物信息学预测和分析结果可为研究在布鲁氏菌致病及机体免疫应答中甲酰基转移酶的结构与功能的关系提供丰富资料。To analyze and predict the structure and characteristics of formyltransferase of Brucella melitensis by bioinformatics in order to guide the experimental research for its biological function and application.Bioinformatics software（DANMAN and Vector NTI）,database（prosite and PDB） and net servers were applied to predict and analyse the structures and biochemical properties of formyltransferase of brucella melitensis.Amino acid composition and domain as well as the secondary structures including regions of helix,sheet,turn,coil of the enzyme were well defined.Flexibility,hydrophilicity and surface accessibility which are always used to predict the B-cell epitopes and were simultaneously displayed.These predicted and analytic results could provide data for understanding the relationship between structure and function of the formyltransferase in the pathogenesis and the immunological response.

关 键 词：羊布鲁氏菌 甲酰基转移酶 生物信息学分析 

分 类 号：Q811.4[生物学—生物工程]