宁夏肉牛杂交改良群体生长激素受体基因的多态性研究  

Study on Polymorphisms of GHR Gene in Improved Hybrid Beef Cattle Groups in Ningxia Province

作　　者：李聚才[1] 张春珍[2] 杨易[2] 于建勇 

机构地区：[1]宁夏农林科学院草畜工程技术研究中心,宁夏银川750002 [2]宁夏大学,宁夏银川750021 [3]泾源县农牧与科技局,宁夏泾源756400

出　　处：《中国畜牧兽医》2011年第7期151-155,共5页China Animal Husbandry & Veterinary Medicine

基　　金：宁夏科技攻关计划项目(5183003);科技人员服务企业行动项目(2009GJG30036)

摘　　要：试验通过对宁夏六盘山区肉牛杂交改良群体生长激素受体(growth hormone receptor,GHR)基因的多态性研究,为宁夏肉牛杂交改良选育提供理论基础。采用PCR-RFLP对该群体70个个体GHR基因多态性进行检测,结果扩增出338 bp的目的片段,PCR产物被限制性内切酶AluⅠ消化后表现出多态性,共检测到2种基因型:AA、AB,其中A为优势等位基因,AA基因型为优势基因型。Polymorphism of the growth hormone receptor（GHR） in improved hybrid beef cattle group from Liupan Mountain area in Ningxia province was investigated to give the rationale for hybrid improvement.Polymerase chain reaction-restriction fragment length polymorphisms（PCR-RFLP） technology was carried out to examine polymorphisms of GHR gene of 70 individuals.PCR products of 338 bp digested by restriction enzyme Alu Ⅰ showed polymorphisms.There were two genotypes of AA and AB.The frequency of A allele was higher than B allele,and the frequency of AA genotype was higher than AB genotype.

关 键 词：肉牛 生长激素受体基因 多态性 

分 类 号：S823.92[农业科学—畜牧学]